摘要
目的探讨结核分枝杆菌T细胞斑点试验(T-SPOT·TB)技术诊断结核性胸膜炎的临床价值。方法选择2015年1月至2016年3月于我院就诊的176例疑似结核性胸膜炎的患者作为研究对象,使用T-SPOT·TB技术检测外周血单个核细胞中对结核分枝杆菌早期分泌靶抗原6(ESAT-6)和/或培养滤液蛋白10(CFP-10)致敏的T细胞,并将T-SPOT·TB技术的检测结果与腺苷脱氢酶(ADA)、PPD皮内试验、胸水结核分枝杆菌聚合酶链反应(TB-DNA)进行对比。结果 T-SPOT·TB技术检测的准确性为92.05%,特异度为92.31%,灵敏度为92.00%,均明显优于TB-DNA、ADA、PPD等检测方法(P<0.05)。结论 TSPOT·TB技术较适用于结核性胸膜炎的诊断中,特异度、灵敏度与准确性均明显高于其他检测方法,值得临床推广应用。
Objective To investigate the clinical value of Myeobacterium tuberculosis T-cell spot （T-SPOT-TB） test in the diagnosis of tuberculous pleurisy. Methods One hundred and seventy-six patients with suspected tuberculous pleurisy in our hospital from January 2015 to March 2016 were selected as the research objects. T-SPOToTB was used to test the mycobacterium tuberculosis early secretory antigentic target 6 （ESAT-6） in peripheral blood mononuclear cell and/or culture filtrate protein 10 （CFP-10） sensitized T cells. Then the results of T-SPOT ＂TB and the results of adenosine deaminase （ADA）, PPD skin test, polymerase chain reaction for pleural effusion myeobaeterium tuberculosis （TB-DNA） were compared. Results The accuracy of T-SPOT＇TB test was 92.05%, specificity was 92.31%, sensitivity was 92.00%, which were significantly better than those of ADA, PPD, TB-DNA test （P〈0.05）. Conclusion T-SPOT ＂TB test is suitable for diagnosis of tuberculous pleurisy, whose specificity, sensitivity and accuracy are significantly higher than other test methods, which is worthy of clinical application.
出处
《临床医学研究与实践》
2017年第24期45-46,共2页
Clinical Research and Practice
