摘要
为了进行猪A-FABP基因的克隆及不同器官组织中的差异表达,试验采用RT-PCR方法从长白猪背最长肌中克隆A-FABP基因的cDNA序列并进行测序比对,分别与人、牛、山羊、家鼠及鸡进行同源性比较,应用半定量RT-PCR方法检测猪心脏、肝脏、脾脏、肺脏、肾脏、背最长肌、腿肌中A-FABP基因的表达。结果表明:成功克隆得到A-FABP基因的cDNA序列,且比对结果为100%,该序列与人、牛、羊的同源性分别为90.98%、89.97%、89.97%,与鸡的同源性为73.68%。半定量RT-PCR结果为A-FABP基因在心脏、肝脏、脾脏、肺脏、肾脏、背最长肌、腿肌器官组织中均有表达,其中背最长肌和腿肌中A-FABP基因的表达量最高,肺脏内的表达量最低,A-FABP基因在动物进化中具有高度保守性,该基因在猪不同器官组织中的表达具有差异性。
In order to investigate the cloning and differential mRNA expression of A - FABP gene in different tissues of swine,in this experiment the cDNA of A - FABP gene from longissimus dorsi muscle of swine was cloned and sequenced by RT - PCR, and was aligned in comparison with human, cattle, goat, house mouse and chicken respectively. The expression of A - FABP mRNA in different tissues （ heart, liver, spleen, lung, kidney, longissimus dorsi muscle and leg muscle） was detected by using semi - quantitative RT - PCR. The results showed that the cDNA of A - FABP gene was successfully cloned, and was in 100% homology with the standard sequence in NCBI database. The A - FABP gene shared about 90.98% ,89.97% ,89.97% homology with people,cattle and goat respectively,while sharing about 73.68% with chicken. The results of semi - quantitative RT - PCR indicated that pig A - FABP mRNA was expressed in all seven tissues （ heart,liver, spleen,lung, kidney, longissimus dorsi muscle and leg muscle） ,with the highest levels were in lnngissimus dorsi muscle and leg muscle and the lowest was in lung. A - FABP gene could be highly conservative during the evolution of swine. The expression level of A - FABP mRNA was variant in different tissues.
出处
《黑龙江畜牧兽医》
CAS
北大核心
2017年第8期106-110,共5页
Heilongjiang Animal Science And veterinary Medicine
基金
转基因生物新品种培育科技重大专项项目(2016ZX08006-003)
山东省现代农业产业技术体系生猪创新团队建设项目(SDAIT-08-13)
