摘要
采用灌根接种法研究内生真菌球毛壳菌(Chaetomium globosum)对油菜根肿病的室内生防作用。结果表明,灌根接种球毛壳菌孢子悬浮液后,油菜体内的过氧化物酶、多酚氧化酶、苯丙氨酸解氨酶的活性均明显提高。球毛壳菌孢子悬浮液灌根接种油菜根部后,根肿病的发病率和病情指数均明显降低,对油菜根肿病的防效为76.88%。通过极性梯度萃取、薄层层析和HPLC从球毛壳菌发酵液中分离纯化活性物质,经质谱鉴定为C16鞘氨醇和植物鞘氨醇。鞘氨醇对根肿菌休眠孢子的萌发有明显的抑制作用,其MIC值小于0.1μg/mL,表明球毛壳菌防治油菜根肿病的机制为诱导系统抗性和次生代谢产物拮抗病原菌2种机制。
The biocontrol effect of endophytic fungi Chaetomium globosumagainst rapeseed clubroot was studied in laboratory.Results showed that after irrigating spores suspension of C.globosum around rapeseed root,the activities of peroxidase,polyphenol oxidase and phenylalanine ammonia lyase were all obviously increased.When the spore suspension of C.globosum was inoculated by irrigation rapeseed rhizosphere soil,the disease incidence and disease index of rapeseed clubroot were significantly decreased.The control efficiency was 76.88%.The active compounds were isolated and purified from C.globosum culture by polarity gradient extraction,thin layer chromatography and HPLC.The active substances were identified as C16 sphingosine and phytosphingosine via LC-MS.The sphingosine obviously inhibited the germination of Plasmodiophora brassicae resting spores.MIC value of sphingosine was lower than0.1μg/mL.The antagonistic mechanisms of C.globosumto P.brassicae were confirmed as induced systemic resistance and secondary metabolites.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2016年第5期58-62,共5页
Journal of Huazhong Agricultural University
基金
国家重点基础研究发展计划("973"计划)项目(2013CB127504-4)
关键词
油菜根肿病
根肿菌
球毛壳菌
鞘氨醇
生防机制
rapeseed clubroot
Plasmodiophora brassicae
Chaetomium globosum
sphingosine
biocontrol mechanism
