摘要
目的研究铁皮石斛叶对亲代和子代雄性SD大鼠精子质量及睾丸组织的影响,为铁皮石斛的安全性评价提供毒理学资料。方法分别对亲代(P)、一代(F1)和二代(F2)SD大鼠设置对照组和低、中、高三个剂量组,每组10只,以喂养方式分别给予含0、2.0、4.0和6.4 g/kg·bw铁皮石斛叶受试物的基础饲料。各组连续给受试物3个月后,测定雄性大鼠睾丸、附睾脏器重和脏体比;在显微镜下观察精子数量、活动率和畸形率;采用苏木精-伊红染色法观察睾丸、附睾组织的病理改变。结果 P、F1、F2各剂量组和对照组大鼠的睾丸重、附睾脏器重、脏体比比较,差异均无统计学意义(P>0.05);P、F1和F2的高剂量组大鼠精子畸形率分别与对照组比较,差异均无统计学意义(P>0.05)。P、F1和F2大鼠睾丸、附睾组织形态无明显改变。结论铁皮石斛叶对P、F1和F2大鼠精子质量和睾丸、附睾组织未见明显不良影响。
Objective To investigate the effects of dendrobium officinale leaves on sperm quality and testicular tissue morphology in parent （P） and offspring （F1, F2） rats. Methods P, F1, F2 rats were fed with dendrobium officinale leaves at the dose of 0, 2.0, 4.0, 6.4g/kg· bw for two generation reproduction, and the testicular and epididymal viscera coefficients, the quantity and quality of sperm were examined and the histopathological assessment was carried out. Results There were no statistical differences in the testicular and epididymal viscera coefficients, sperm quantity, sperm motilily rate and sperm malformation rate compared with the control group in P, F1, F2 rats （P〉0.05） . There were no differences between 0 and 6.4 g/kg ·bw groups on the testicular and epididymal tissue morphology in P, F1, F2 rats. Conclusion Dendrobium officinale leaves didn＇t show obvious adverse effects on sperm quality and testicular tissue morphology in P, F1, F2 rats.
作者
傅剑云
宋燕华
何佳维
朱周靓
宋松
夏勇
FU Jian-yun SONG Yan-hua HE Jia-wei ZHU Zhou-jing SONG Song XIA Yong(Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang, 310051, China)
出处
《预防医学》
2017年第8期782-785,789,共5页
CHINA PREVENTIVE MEDICINE JOURNAL
关键词
铁皮石斛叶
大鼠
精子
睾丸
Dendrobium officinale
Leaf
Rat
Sperm
Testis
