摘要
目的探讨非小细胞肺癌原发部位和转移淋巴结中程序性细胞死亡因子1配体(programmed death-ligand 1,PD-L1)的表达水平及其临床特征和预后情况。方法收集病理组织学确诊的55例非小细胞肺癌手术标本和40例超声支气管内镜活检的淋巴结标本。采用免疫组织化学法和实时荧光定量PCR法检测PD-L1蛋白和mRNA的表达。采用生存分析探讨PD-L1蛋白表达和肺癌患者预后的关系。结果 PD-L1蛋白在非小细胞肺癌原发部位的阳性率是23.6%(13/55)。PD-L1表达阳性率在病理类型(腺癌vs鳞癌,P=0.047)和病理分期(Ⅰ期vsⅡ~Ⅲ期,P=0.025)方面差异均具有统计学意义。PD-L1蛋白表达阳性患者平均无复发生存时间较短(P=0.049)。PD-L1 mRNA在原发部位鳞癌组织中的表达高于腺癌组织(P=0.017),且随病理分期的增加而升高(P=0.029)。原发部位和转移淋巴结中PD-L1蛋白和mRNA的表达差异均无统计学意义(均P>0.05)。PD-L1蛋白在转移淋巴结中的表达在患者年龄、性别、吸烟状态、病理类型、分化程度、病理分期和总生存方面差异均无统计学意义(均P>0.05)。结论 PD-L1可促进非小细胞肺癌细胞的浸润和转移,在原发部位鳞癌组织中的表达高于腺癌组织。
Objective To investigate the expression of programmed death-ligand 1（ PD-L1） and its association with the clinical characteristics and prognosis of the primary tumor and metastatic lymph node of non-small-cell lung cancer（ NSCLC）. Methods The surgical samples of 55 cases of pathologically proved NSCLC,as well as 40 metastatic lymph node samples from endobronchial ultrasonography biopsy were collected. The expressions of PD-L1 protein and mRNA were detected by immunohistochemistry and quantitative real-time PCR,respectively. The relationship between PD-L1 protein level and the prognosis of NSCLC patients was analyzed. Results The positive rate of PD-L1 protein expression in the primary lesion of NSCLC was 23. 6%（ 13/55）,which varied significantly in patients of different pathological types（ adenocarcinoma vs squamous carcinoma,P = 0. 047） or different pathological stages（ stage Ⅰ vs stageⅡ-Ⅲ,P = 0. 025）. Moreover,NSCLC patients with positive PD-L1 expression tended to have short recurrence-free survival time（ P = 0. 049）.PD-L1 mRNA level was also higher in primary squamous carcinoma than in primary adenocarcinoma（ P = 0. 017）,and was increased as the pathological stage advanced（ P = 0. 029）. Moreover,the levels of PD-L1 protein and mRNA were similar between the primary lesions and metastatic lymph nodes of NSCLC（ P〉0. 05）. Finally,the expression of PD-L1 protein tumors in metastatic lymph node tumors was not influenced by gender,age,smoking status,pathological types,pathological differentiation,pathological stages,or overall survival（ all P〉0. 05）. Conclusion PD-L1 may drive the invasion and metastasis of NSCLC cells,and showed higher expression in primary squamous than primary adenocarcinoma.
作者
李佳
张菁
李晓
马胜林
张仕蓉
Li Jia Zhang Jing Li Xiao(Departments of Oncology, Hangzhou First People＇s Hospital, Nanjing Medical University, Hangzhou 310006, China Department of Pathology, Hangzhou First People＇s Hospital, Nanjing Medical University, Hangzhou 310006, China)
出处
《实用肿瘤杂志》
CAS
2017年第4期332-336,共5页
Journal of Practical Oncology
关键词
癌
非小细胞肺/病理学
淋巴转移
膜糖蛋白类/代谢
T淋巴细胞/免疫学
配体
基因表达
免疫组织化学
聚合酶链反应
carcinoma
non-small-cell lung/pathology
lymphatic metastasis
membrane glycoproteins/metabolism
T-lymphocytes/immunology
ligands
gene expression
immunohistochemistry
polymerase chain reaction
