摘要
目的探讨非小细胞肺癌(non-small cell lung cancer,NSCLC)中程序性死亡受体配体1(programmed death receptor ligand1,PD-L1)表达和调节性T细胞(regulatory T cell,Treg)浸润的关系及临床意义。方法采用免疫组化法检测78例NSCLC组织中PD-L1表达和Treg浸润情况,分析二者之间的相关性及与NSCLC临床病理特征的关系。结果肺癌组织中PD-L1的表达显著高于癌旁组织(52.5%vs 6.4%),肺癌组织中Treg浸润亦明显高于癌旁组织[(18.63±16.67)个/HPF vs(2.96±2.97)个/HPF]。晚期肺癌组织中PD-L1的表达高于早期肺癌组织(70.0%vs 41.7%),晚期肺癌组织中Treg浸润亦明显高于早期肺癌组织(73.3%vs 35.4%),有淋巴结转移组中PD-L1表达和Treg浸润均明显高于无淋巴结转移组,PD-L1表达和Treg浸润程度与淋巴结转移及临床分期相关(P<0.05)。肺癌组织中PD-L1表达和Foxp3+Treg的浸润密度呈正相关(rs=0.611,F=78.82,P=0.023)。结论肺癌微环境中PD-L1表达和Treg浸润呈正相关,二者可能共同参与肺癌的进展和免疫逃逸。
Purpose To investigate relationship between PD-L1 molecule expression and Treg infiltration in non-small cell lung cancer （NSCLC） tissue and to explore their clinical significance. Methods Immunohistochemistry was used to detect the PD-L1 molecules expression and Treg infiltration of 78 NSCLC tissues. The relationship among PD-L1 expression, Treg infiltration and clinic-pathological parameters was analyzed in the patients. Results PD-L1 molecule was highly expressed in lung cancer tissues than adjacent tissues （52. 5% vs 6. 4% ）, and same to Foxp3＋ Treg （ 18.63 ± 16. 67）/HPF vs （2.96±2. 97）/HPF. There was close relationship between PD-L1 expression and Treg infiltration with lymph node metastasis and clinical stage of patients, but was no statistical correlation with patient＇ s age, gender, histological type and degree of differentiation of the tumor cells. PD-L1 expression was significantly higher in advanced stage than that in early stage （70. 0% vs 41.7% ） and same to Treg infiltration （73.3% vs 35.4% ）. There were also signif- icantly higher infiltration with lymph node metastasis than that without metastasis. In addition, PD-L1 molecule expression and Foxp3＋ Treg infiltration were positively correlated （ r, = 0. 611, F = 78.82, P = 0. 023 ）. Conclusion There was strong relationship among PD-LI expression, Treg infiltration and disease progress in lung cancer patients, and they possibly participate in the progression and immune escape of lung cancer.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2015年第4期418-421,425,共5页
Chinese Journal of Clinical and Experimental Pathology
基金
国家自然科学基金(31270940
81401943)
国家临床重点专科建设项目
江苏省自然科学基金(BK2012606)
江苏省科技专项(BL2012023)
苏州市科技计划项目(SYS201462)
