摘要
目的:检测小鼠黑色素瘤细胞中核转录因子Foxp3的表达。方法:用TRIzol试剂提取小鼠黑色素瘤细胞RNA,通过实时荧光定量PCR检测小鼠黑色素瘤细胞中Foxp3 mRNA的表达,通过Western印迹和流式细胞术检测小鼠黑色素瘤细胞中Foxp3蛋白的表达,通过免疫荧光检测小鼠黑色素瘤细胞中Foxp3分子的表达。结果:在小鼠黑色素瘤细胞中存在Foxp3的mRNA转录和分子表达,免疫荧光显示Foxp3定位于黑色素瘤细胞的胞核及核周部位。结论:证实了小鼠黑色素瘤细胞表达Foxp3,将为临床黑色素瘤的免疫治疗提供新的靶标和治疗策略。
Objective： To detect the expression of nuclear transcription factor Foxp3（forkhead/winged helix tran scription factor） in the mouse melanoma cells. Methods： The RNA was extracted using TRIzol from mice melanoma cells, and the expression of Foxp3 mRNA in the mouse melanoma cells was detected by realtime fluores cence quantitative PCR, Western blot and flow eytometry were used to detect the expression of Foxp3 protein, and discovered the Foxp3 molecules by immunofluoreseence. Results： The Foxp3 mRNA and protein were detected in the mouse melanoma cell lines. The subcellular distribution of the Foxp3 molecule was confirmed in the nucleus and cytoplasm of the melanoma cell. Conclusion： This project confirmed the expression of Foxp3 in the murine melanoma cell, and provide new targets and therapeutic strategies for the melanoma clinical immunotherapy.
出处
《生物技术通讯》
CAS
2012年第2期198-200,共3页
Letters in Biotechnology
基金
解放军总医院第一附属医院2009年度重大项目(ZD200903)
关键词
核转录因子
FOXP3
黑色素瘤
表达
鉴定
nuclear transcription factor
forkhead/winged helix transcription factor（Foxp3）
melanoma
expres-sion
identification
