摘要
目的甲状腺癌是内分泌系统最常见的恶性肿瘤之一,甲状腺腺瘤是最常见的甲状腺良性肿瘤,研究发现甲状腺良、恶性肿瘤在形成过程中存在多种基因表达的差异。本研究拟探讨生物节律基因(circadian locomotor output cycles kaput,CLOCK)在甲状腺癌与腺瘤组织中的表达及其临床意义。方法系统收集2012年3月—2015年3月在徐州医科大学附属淮海医院普外科手术切除的甲状腺癌标本74例及甲状腺腺瘤标本55例,应用免疫组化方法检测CLOCK表达水平,并分析其与临床病理特征的关系。结果甲状腺癌、甲状腺腺瘤组织中CLOCK的阳性表达率分别为68.9%(51/74)和32.7%(18/55),两者差异有统计学意义(P<0.001)。甲状腺癌组织CLOCK表达量为4.2±3.8,甲状腺腺瘤组织CLOCK表达量为2.1±2.0,差异有统计学意义(P<0.001)。CLOCK在有淋巴结转移的癌组织中阳性表达率明显高于无淋巴结转移的癌组织(P=0.012),且CLOCK阳性表达率随肿瘤体积增大而提高(P=0.028),而且CLOCK阳性表达率随TNM分期的提高而提高(P=0.023)。结论 CLOCK表达上调不仅参与甲状腺癌的形成过程,而且在肿瘤的侵袭、转移等演进过程中发挥着重要作用。
Objective Thyroid adenocarcinoma is one of the most common malignancies in the endocrine system, while thyroid adenoma is the most common thyroid benign tumor. Some research has showed the difference in the gene expres- sion between benign and malignant thyroid tumor. The aim of this study was to investigate the expression and clinical sig- nificance of biological rhythm gene CLOCK in thyroid carcinoma and adenoma. Methods Total 74 cases of thyroid carci- noma and 55 cases of thyroid adenoma were collected from March ,2012 to March ,2015 in our hospital. The expression of CLOCK was detected by immunohistoehemical method. We analyzed its relationship with clinicopathologieal features. Resuits The positive rates of CLOCK in thyroid carcinoma and thyroid adenoma were 68.9% （51/74） and 32.7% （ 18/ 55 ） ,respectively,the difference was significant（P 〈 0. 001 ）. The expression of CLOCK in thyroid carcinoma was 4.2 ± 3.8, and in thyroid adenoma was 2.1 ± 2.0, there was significant difference （ P 〈 0.001 ）. The positive expression rate of CLOCK in cancer tissues with lymph node metastasis was significantly higher than that without lymph node metastasis （P = 0. 012 ）. The positive expression rate of CLOCK was significantly correlated with the tumor size（ P = 0. 028 ）, and the CLOCK positive expression rate increased with TNM stage （ P = 0. 023 ）. Conclusion Up-regulation of CLOCK is not only involved in the formation of thyroid carcinoma, but also plays an important role in the process of tumor invasion and metastasis.
作者
刘冬良
华明
周峰
韩昌
牛万成
李德本
张召辉
LIU Dong-liang HUA Ming ZHOU Feng et al(Department of General Surgery, Huaihai Hospital Affiliated to Xuzhou Medical University,Xuzhou, Jiangsu 221004, China)
出处
《中华全科医学》
2017年第10期1675-1677,1785,共4页
Chinese Journal of General Practice
基金
南京军区医学科技创新课题项目(15ZD-013)
