摘要
[目的]观察磁化活血通络灵液对雌性去势大鼠骨密度、骨组织形态学、骨生物力学影响,探究其对快速骨流失的影响和骨质疏松的防治作用。[方法]将84只SPF级SD雌性大鼠随机分为空白对照组、去势对照组、去势后服用阿仑膦酸钠组[7 mg/(kg·w)]、去势后涂磁化活血通络灵药液组[1 mL/(100g·d)]、加磁1组(120 mT)、加磁2组(180 mT)、加磁3组(220 mT),每组12只。除空白对照组外,均采用摘除卵巢的方法复制快速骨流失大鼠模型,造模后第3天,各组分别施加相应的干预因素。术后5周,第1次心脏取血。每组取血6~8只,用于骨代谢指标检测。术后6周,各实验组分取6只大鼠处死,行骨组织形态计量学检测。术后18周,按前法行第2次心脏取血,同前法检测。处死后分取双下肢股骨,用于骨密度、骨组织形态学、骨生物力学检测。[结果]与去势对照组比较,大鼠去势经磁化活血通络灵液治疗后,从骨计量学结果、骨密度测量、Micro-CT以及力学三点弯曲试验显示,其对改善松质骨骨小梁细微结构和提高皮质骨的力学性能等方面作用明显(P<0.05)。[结论]实验表明,磁化活血通络灵液对延缓骨量丢失,尤其在骨密度、骨组织形态学、骨生物力学方面对骨质疏松症有一定的治疗效果。
[Objective] To observe the effect of magnetized Huoxue Tongluo Ling （MHTL） on the bone mineral density,bone histomorphology and bone biomechanics of female ovariectomized rats, and to explore its effect on rapid bone loss and prevention and treatment of osteoporosis. [Methods] Eighty-four SPF grade SD magnetic rats were randomly divided into blank control group and ovariectomized control group, ovariectomized and alendronate sodium group,ovariectomized and MHTL group, emagnetized group 1, magnetized group 2, magnetized group 3, with 12 each group. Besides the blank control group, the rats were divided into two groups according to the method of ovariectomy. On the third day after modeling, the corresponding intervention factors were applied to each group. Five weeks after surgery,we took collect specimens of blood for bone metabolism test, with 6~8 rats each group, for bone metabolism indicators. Six rats were sacrificed at 6 weeks after operation, and the bone histomorphometry was performed. Eighteen weeks after surgery,according to the law, we took collect specimens of blood again, with the former method of detection. After the execution, it was took the lower limb femur for bone mineral density, bone histomorphology,bone biomechanics [Results] Compared with the control group, the results from the rats treated byMHTL,were obtained from bone metrology,bone density measurement,Micro-CT and mechanical three-point bending test （ P〈0.05）. It was found that it could improve the microstructure of cancellous bone trabecula and improve the mechanical properties of cortical bone. From the large amount of experimental data obtained from bone metrology, bone density measurement, Micro-CT and mechanical three-point bending test, compared with the ovariectomized group. The rats, treated with magnetized Huoxue Tongluo ling decotion was found that its microstructure of cancellous bone trabecula and the mechanical properties of cortical
作者
高翔
刘道矩
GAO Xiang LIU Dao-Ju(Tianjin Occupational Disease Prevention and Control Hospital, Tianjin 300011, China)
出处
《天津中医药大学学报》
CAS
2017年第4期286-290,共5页
Journal of Tianjin University of Traditional Chinese Medicine
基金
天津市中医药管理局中医
中西医结合科研专项资助项目(13138)
关键词
磁场
活血通络灵液
大鼠
骨丢失
magnetic field
Huoxue Tongluo Ling decotion
rats
bone loss
