摘要
目的了解重庆地区Rh阴性血清学表型分布。方法采用微板快速检测法对本站2015年1-12月份129 315份献血者标本进行Rh D抗原初筛;初筛阴性者采用木瓜酶法和间接抗球蛋白法进行确认;确认阴性者用盐水试管法对Rh因子C、c、E、e进行表型鉴定。结果 129 315名献血者中共确认Rh D阴性603例,其中A型135例(22.39%),B型170例(28.2%),O型170例(28.2%),AB型128例(21.23%);Rh表型频率ccdee(49.92%)>Ccdee(39.47%)>CCdee(6.97%)>ccdee(2.82%)>Ccd Ee(0.83%)。结论通过微孔法等技术可检测和建立Rh D阴性稀有血型库并明确其表型。
Objective To investigate the distribution frequencies of Rh（D） negative phenotype in Chongqing.Methods129 315 samples collected from January to December 2012 had their Rh（D） phenotypes initially screened by microplate test,papain test and indirect anti-globulin test,which were also used to further confirmed the negative phenotype.The phenotypes of C,c,E,e were detected by brine method.Results 603 cases of Rh（D） negative phenotype were confirmed in129 315 blood donors：A 135（22.39%）,B170（28.2%）,O 170（28.2%）,AB 128（21.23%）.The frequency of Rh,ccdee（49.92%）〉Ccdee（39.47%）〉CCdee（6.97%）〉ccdee（2.82%）〉Ccd Ee（0.83%）.Conclusion The establishment of a Rh（D） negative blood donors bank in Chongqing and the confirmation of the phenotypes is made possible by utilizing technology such as microporosity plate.
出处
《中国输血杂志》
北大核心
2017年第6期629-630,共2页
Chinese Journal of Blood Transfusion
关键词
RH血型
抗原
输血
Rh phenotype
antigen
blood transfusion
