摘要
目的以顺铂为主的非小细胞肺癌化疗方案不断更新,目前研究旨在寻求疗效的放大及毒副作用的缩小。黄腐酚被证明有抗癌及保护正常细胞的作用。本实验主要探讨黄腐酚的不同给药方式对顺铂诱导人非小细胞肺癌细胞株H1650凋亡的影响。方法采用CCK-8法检测黄腐酚、顺铂单用及先用低浓度黄腐酚干预,后补入顺铂对H1650细胞增殖的影响;使用流式细胞术分别分析先用黄腐酚后用顺铂、先用顺铂再补入黄腐酚2种干预方式对H1650细胞凋亡率或存活率的影响。结果 CCK-8检测结果显示,2μmol/L黄腐酚干预H1650细胞48h后,继续给予20、40和80μmol/L顺铂干预24h,抑制细胞增殖率分别为(13.16±1.06)%、(19.14±1.67)%和(28.17±2.79)%,均低于同浓度顺铂单纯干预24h组的(18.96±3.02)%、(23.28±1.43)%和(39.79±3.44)%,P<0.05。流式细胞术检测结果显示,10、20和40μmol/L顺铂干预细胞24h后,继续给予7[(85.66±3.19)%、(74.62±2.98)%和(59.55±2.70)%]或14μmol/L[(82.04±2.88)%、(76.50±3.11)%和(61.76±3.01)%]黄腐酚干预24h,其细胞存活率明显高于同浓度顺铂单纯干预48h组的(72.60±2.69)%、(56.98±3.80)%和(44.15±2.01)%,P<0.05。3μmol/L黄腐酚干预细胞24h,弃培养液,更换10、20和40μmol/L顺铂再干预24h,其细胞凋亡率分别为(29.79±3.78)%、(30.53±3.89)%和(39.48±4.98)%,明显高于顺铂单纯干预24h组的(17.21±3.01)%、(14.53±2.99)%和(18.88±4.20)%,P<0.05。结论黄腐酚直接有效保护顺铂对H1650细胞的杀伤,但低浓度黄腐酚的前期干预间接提高了顺铂对H1650细胞的凋亡率。
OBJECTIVE Cisplatin-based chemotherapy for non-small cell lung cancer （NSCLC） has been continu ously updated to improve therapeutic efficacy and reduce side effects. It has been shown that xanthohumol has the effects of anticancer and protecting normal cells. In this study, we investigated the effect of xanthohumol on the apoptosis of cisplatin-induced human NSCLC cell line H1650 under different ways of administration. METHODS The effect of xanthohumol alone, cisplatin alone and cisplatin following low concentration of xanthohumol on the proliferation of H1650 cells was detected using CCK-8 method. The effect of two administration methods, namely cisplatin following xanthohumol and xanthohumol following cisplatin,on the apoptosis rate or survival rate of H1650 ceils was analyzed through flow cytometry （FCM）. RESULTS Determined by CCK-8/FCM,after H1650 cells were intervened by 2μmol/L xanthohumol for 48 h, and then by 20,40 and 80μmol/L cisplatin for 24 h,the inhibition proliferation rate[（13.16±1.06） %, （19.14±1.67） %, （28.17±2.79） %] was lower than that by the same concentration of cisplatin alone for 24 h[（18.96±3.02） %,（23.28±1.43）% ,（39.79±3.44）% ,P〈0.05]. After H1650 cells were intervened by 10,20 and 40 μmol/L cisplatin for 24 h, and then by 7 or 14 μmol/L xanthohumol for 24 h, the survival rate [（85. 66 ± 3. 19） %, （82.04 ± 2.88）%; （74. 62 ± 2.98） %,（76.50±3.11）%;（59.55±2.70）%,（61.76±3.01）%] of cells was significantly higher than that by the same concentration of cisplatin alone for 48 h [（72.60±2.69）%, （56. 98±3.80）%, （44. 15±2.01）% ,P〈0. 05]. After H1650 cells were intervened by 3 μmol/L xanthohumol for 24 h, and then the culture medium was removed and 10, 20 and 40 μmol/L cisplatin were used to intervene 24 h, the apoptosis rate [（29.79±3.78）%, （30.53 ± 3.89）%, （39.48 ± 4.98）%] was markedly higher than that intervened by cisplatin alone for 24 h [（17.21±3.01）%, （14.53±2.99）%, （18.88±4
作者
李晨龙
张永东
郭红云
赵勤
刘启明
苏海翔
LI Chen-long ZHANG Yong dong GUO Hong-yun ZHAO Qin LIU Qi-ming SU Hai-xiang(Department of Geriatrics, The Directly Affiliated Hospital of Wuwei Occupational College , Wuwei 733000, P. R. China Translational Medicine Research Center ,Gansu Provincial Academic Institute for Medical Research/Tumor Hospital of Gansu Province ,Lanzhou 730050 ,P. R. China College of Chemistry and Life Sciences ,Changchun University of Technology ,Changchun 130012 ,P. R. China)
出处
《中华肿瘤防治杂志》
CAS
北大核心
2017年第14期960-965,共6页
Chinese Journal of Cancer Prevention and Treatment
基金
甘肃省中医药管理局科研课题(GKZ-2015-9)
