摘要
复方牛黄消炎胶囊作为清热解毒、镇静消炎的经典复方,朱砂是其发挥药效的主要成分之一。该实验采用HNO_3-HClO_4消解体系结合冷原子-原子荧光光谱(CV-AFS)分析技术检测大鼠全血中的汞元素。研究大鼠灌胃给药不同剂量朱砂和复方牛黄消炎胶囊后,全血中汞的药代动力学行为,探讨中药复方配伍对汞体内代谢过程的影响。研究发现大鼠灌胃高、中、低3个剂量朱砂和复方牛黄消炎胶囊后,全血中汞均呈非线性药代动力学特征。大鼠灌胃复方牛黄消炎胶囊后,与单味朱砂相比,全血中汞元素的t_(max),Ke,CL/F显著降低,Cmax/dose,AUC/dose,t_(1/2)显著增加,V_z/F,MRT不存在显著差异,提示经复方配伍后,汞元素在全血中吸收的总量显著增加且吸收速度加快,但消除速度减慢,提示长期服用可能会引起汞在体内的蓄积。
Fufang Niuhuang Xiaoyan capsule was a classical compound prescription with the efficacy of heat-clearing, detoxifica- tion, sedation and anti-inflammation, with cinnabaris as one of its active ingredients. The study focuses on the pharmacokinetics of mercury in rats after oral administration of cinnabaris and Fufang Niuhuang Xiaoyan capsule, in order to explore the effect of combined traditional Chinese medicines on mercury metabolism. In this study, the method of nitric-perchloric acid digestion system coupled with cold atomic-atomic fluorescence spectroscopy （CV-AFS） was adopted to accurately determine mercury in whole blood of rats. Fufang Niuhueng Xiaoyan capsule had three dose schemes of oral administration, namely equivalent clinical dose, 3 times of equivalent clinical dose and 10 times of equivalent clinical dose; And the doses of oral administration of cinnabaris was calcul_ated according to that of Fu- fang Niuhuang Xiaoyan capsule. SPF grade healthy SD rats were fasted overnight before the oral administration with cinnabaris suspen- sion （or Fufang Niuhuang Xiaoyan capsule suspension）. After oral administration of different doses of cinnabaris, no obvious changes in tmax and MRT were observed, while Cmax=/dose, AUC0-48 h/dose and AUC0-∞/dose decreased with the increase in dose, indicating that total mercury absorption in body was declining. As the dose increased, Ko, CL/F decreased, and t1/2 increased, indicating that the elimination slowed down, and mercury metabolism showed non-linear dynamic characteristics within a certain range of dose （22-220 mg · kg-1 ）. The total mercury metabolism in the whole blood of rats after oral administration with different doses of Fufang Niuhuang Xi- aoyan capsule also showed non-linear dynamic characteristics. The results were correlated with the low solubility of cinnabaris in the body. Compared with cinnabaris, Fufang Niuhuang Xiaoyan capsule showed no obvious changes in V/F and MRT, while Ko, CL/F, tmaxdecreased, and t1/2, Cmax/dose, AUC0-48h/dose,
作者
宋晓妮
陆宇婷
杨丹亿
汪生
杭太俊
宋敏
SONG Xiao-ni LU Yu-ting YANG Dan-yi WANG Sheng HANG Tai-jun SONG Min(Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2017年第14期2779-2783,共5页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81473176)
