摘要
目的探索5-羟色胺1A(5-HT1A)受体激动剂8-OH-DPAT对青春期大鼠病理性攻击行为及对前额叶皮质、海马内脑源性神经营养因子(BDNF)和胶质细胞源性神经营养因子(GDNF)的影响。方法出生后21d的雄性SD大鼠42只随机分为6组(n=7):模型组、正常组、模型+药物组、模型+生理盐水(NS)组、正常+药物组、正常+NS组。模型组、模型+药物组及模型+NS组采用早年慢性应激建立病理性攻击模型,余3组正常饲养。建模成功后对模型+药物组、正常+药物组及模型+NS组、正常+NS组分别行2周的8-OH-DPAT(0.5 mg/kg)或生理盐水(2mL/只)腹腔注射。用居住-入侵实验检测大鼠攻击行为,蛋白质印迹分析检测大鼠脑前额叶皮质及海马内BDNF、GDNF蛋白表达水平。结果与正常组比较,模型组攻击潜伏期缩短(P<0.01)、攻击持续时间及攻击总数增加(P<0.01)。模型+药物组攻击潜伏期较模型+NS组延长(P<0.05);模型+药物组攻击持续时间较正常+药物组增加(P<0.05);药物干预后攻击总数组间比较差异无统计学意义(P>0.05)。模型组前额叶皮质、海马内BDNF及GDNF表达水平均低于正常组(P<0.01),模型+NS组较正常+NS组降低(P<0.05,P<0.01),模型+药物组较模型+NS组升高(P<0.05,P<0.01)。结论前额叶皮质及海马内BDNF、GDNF可能与早年慢性应激所致青春期大鼠病理性攻击行为的发生有关。5-HT1A受体激动剂可上调前额叶皮质及海马内BDNF、GDNF的蛋白表达,并在一定程度上减轻早年慢性应激所致青春期大鼠病理性攻击行为。
Objective To explore the effect of serotonin 1A（5-HT1A）receptor agonist 8-OH-DPAT on pathological aggressive behavior,brain-derived neurotrophic factor（BDNF）and glial cell-derived neurotrophic factor（GDNF）in prefrontal cortex and hippocampus in pubertal rats.Methods Forty-two 21-day-old male SD rats were randomly divided into 6groups：model group,normal group,model＋8-OH-DPAT group,model＋normal saline（NS）group,normal＋8-OH-DPAT group and normal＋NS group,with 7rats in each group.The rats in the model,model＋8-OH-DPAT and model＋NS groups were given a series of early chronic stresses to establish the pathological aggressive animal model,and the other 3groups were fed normally.Then the rats in the model＋8-OH-DPAT and normal＋8-OHDPAT groups were injected intraperitoneally with 8-OH-DPAT（0.5 mg/kg）,while the rats in the model＋NS and normal groups were administered with 2mL of NS.We determined the aggressive behaviors of the rats through residentintruder test and detected the protein expressions of BDNF and GDNF in prefrontal cortex and hippocampus by Western blotting.Results（1）Compared with the normal group,the latency in the first attack in the model group was significantly shorter（P〈0.01）,while duration of attack manifestations and total attacks were significantly higher（P〈0.01）.The latency of the first attack in the model＋8-OH-DPAT group was significantly higher than that in the model＋NS group（P〈0.05）;the duration of attack manifestations in the model＋8-OH-DPAT group was significantly increased compared with the normal＋8-OH-DPAT group（P〈0.05）;and there was no significant difference in the total attacks among the groups（P〉0.05）.（2）The protein expressions of BDNF and GDNF in the prefrontal cortex and hippocampus in the model group were significantly lower than those in the normal group（P〈0.01）,but they were significantly decreased in the model＋NS group compared with the normal＋NS group（P〈0.05,P〈0.01）and were significant
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2017年第7期885-890,共6页
Academic Journal of Second Military Medical University
基金
重庆市自然科学基金(CSTC
2011jjA10104)
重庆医科大学国家自然科学预研资助项目(NSFYY201111)~~
