摘要
目的采用先进的样品前处理技术、利用气相色谱-串联质谱法建立14种植物生长调节剂(4-氯苯氧乙酸、2,4-二氯苯氧乙酸、α-萘乙酸、β-萘乙酸、吲哚乙酸、吲哚丁酸、2,4-D乙酯、2,4-D丁酯、萘乙酰胺、整形素、多效唑、烯效唑、激动素、6-苄基腺嘌呤)的高通量检测技术。方法样品经前处理后采用DB-5 MS(30 m×0.25 mm,0.25μm)色谱柱程序升温分离,质谱多反应监测模式(MRM)检测,外标法定量。结果 14种植物生长调节剂在相应的浓度范围具有良好的线性关系(r≥0.997 8),各组分检出限在0.02μg/kg^10.00μg/kg。14种植物生长调节剂加标回收率为54.8%~131.6%,相对标准偏差为2.39%~6.38%。结论建立了14种植物生长调节剂的气相色谱-串联质谱的检测方法。方法快速、准确,检出限、回收率和相对标准偏差均符合国内外对植物生长调节剂残留检测的要求。
Objective To develop a high-throughput method for the determination of 14 plant growth regulators including 4-Chlorophenoxyacetic acid,（ 2,4-Dichlorphenoxy） acetic acid,1-Naphthyl acetic acid,2-Naphthaleneacetic acid,3-indolyl-acetic acid,4-（ 3-indolyl）-butyric acid,2,4-D-ethyl ester,2,4-D-1-butyl ester,1-Naphthyl acetamide,chlorflurenol-methy,Paclobutrazol,uniconazole,6-Furfurylaminopurine,6-Benzylaminopurine though the advanced pretreatment methods and gas chromatography tandem mass spectrometry（ GC-MS/MS）. Methods The sample was separated by DB-5MS column（ 30 m × 0. 25 mm,0. 25 μm） using programmed temperature. MS was conducted in multiple reaction monitoring（ MRM） mode for detection,and the external standard method was used for quantification. Results A good linearity was obtained in wide range and the correlation coefficients（ r） were ≥ 0. 997 8. The detection limits were within 0. 02 μg/kg-10. 00 μg/kg. The recoveries were within 54. 8%-131. 6%. The relative standard deviations were within 2. 39%-6. 38%.Conclusion The detection method of 14 plant growth regulators using GC-MS/MS was set up. The method was fast and precise. The detection limit,recovery and the standard deviation can meet the standards of plant growth regulators residue requirement at home and abroad.
出处
《中国卫生检验杂志》
CAS
2017年第13期1867-1871,共5页
Chinese Journal of Health Laboratory Technology
