摘要
目的应用PCR芯片技术检测壬基酚(NP)暴露对仔鼠海马组织差异基因的表达,探讨NP神经毒性的机制。方法将交配成功的孕鼠根据妊娠日期分层后随机分配两组,即对照组(溶剂花生油)和NP暴露组[NP 200 mg/(kg·d)],每组7只,NP暴露时间为受孕第6天至出生后21 d,提取NP暴露组和对照组出生21 d仔鼠海马组织m RNA,应用大鼠神经毒理PCR芯片有针对性地分析暴露NP仔鼠模型海马组织的差异表达基因。结果与对照组相比,NP暴露组共有7个差异表达基因,上调超过2倍的基因有2个:Gal、Tph1;下调超过2倍的基因有5个:Camk2d、Casp7、Gch1、Trpm1、Tyrp1。其中,与凋亡相关的基因为Casp7、Gch1;代谢相关基因为Gal、Tph1、Tyrp1;离子转运相关基因为Camk2d、Trpm1。结论 NP能影响仔鼠神经系统的发育与功能,可能与神经元物质代谢、细胞凋亡、离子转运等相关基因表达改变密切相关。
Objective To investigate the differential gene expression in hippocampus of nonylphenol（NP） treated and control weaning rats and to screen part of neurotoxicity-related differential expression genes with PCR array technology for further verification. Methods Establishing animal models for NP exposure[200 mg/（kg·d）] during gestation and lactation. mRNA of hippocampus in NP-treated group and control group were isolated at 21 dafter birth and the differential gene expression was exam-ined by PCR array technology. Results PCR array showed that the expression levels of 2 genes（Gal,Tph1） increased by double or more compared to those of the control group and the expression levels of 5 genes（Camk2d,Casp7,Gch1,Trpm1,Tyrp1）decreased by double or more compared to those of the control group.The above up and down-regulated genes were involving in metabolism-related genes,apoptosis-related genes and ion channel-related genes. Conclusion Exposure to NP during gestation and lactation might involve in influencing metabolism-related,apoptotic factor-related and ion channel-related differential gene expression in hippocampus neurons,which might have effects on interfering the function and development of CNS in pups.
出处
《现代医药卫生》
2017年第14期2081-2083,共3页
Journal of Modern Medicine & Health
基金
国家自然科学基金(81360439
81560527)
贵州省卫生厅基金(gzwjkj2016-1-045)
贵州省优秀青年基金(黔科合平台人才[2016]5612)
贵州省教育厅大学生创新创业基金(2017)
遵义医学院大学生创新基金(20163718)
关键词
海马
聚合酶链反应
生物传感技术/仪器和设备
酚类/药理学
仔鼠
Hippocampus
Polymerase chain reaction
Biosensing techniques/instrumentation
Phenols/pharmacology
Weaning rat
