摘要
目的探讨micro RNA-196a(miR-196a)对食管癌预后的评估价值及其生物学行为的调控机制。方法收集120例行食管癌根治性手术切除的食管癌组织及癌旁组织,逆转录聚合酶链反应(RT-PCR)检测组织miR-196a表达水平,分析miR-196a表达与食管癌患者临床资料的关系。对食管癌患者进行随访,记录患者总生存期(OS)和无病生存期(DFS);以OS和DFS作为评价指标,采用单变量和多变量Cox比例风险模型评价患者预后的影响因素。分别采用miR-196a mimic、NC-mimic、miR-196a inhibitor、NC-inhibitor转染食管癌TE1细胞,MTT实验检测细胞增殖能力,Transwell实验检测细胞侵袭能力,细胞划痕实验检测细胞迁移能力,Western blot检测细胞ANXA1、NTN4、HMGA2、HOXB8蛋白表达。结果 miR-196a在食管癌组织中表达水平高于癌旁组织(P<0.05);在TE1细胞中,miR-196a mimic组miR-196a表达水平高于NC-mimic组,miR-196a inhibitor组miR-196a表达水平低于NC-inhibitor组(P<0.05),证实细胞转染实验成功。将食管癌患者按照miR-196a表达分为miR-196a高表达组51例和低表达组69例,miR-196a表达与年龄、性别、分化程度、N分期、肿瘤位置等无关(P>0.05),随着T分期、TNM分期和肿瘤直径增加,miR-196a高表达率升高(P<0.05)。生存分析显示,miR-196a高表达患者总生存期(P=0.015)和无病生存期(P=0.017)低于miR-196a低表达者;单因素和多因素分析显示,T分期、miR-196a表达是影响患者总生存期和无病生存期的的独立危险因素(均P<0.05)。MTT实验结果显示,第48~120 h,miR-196a mimic组吸光度值高于NC-mimic组,miR-196a inhibitor组吸光度值低于NC-inhibitor组(P<0.05);Transwell小室实验显示,miR-196a mimic组穿膜细胞数量高于NC-mimic组,miR-196a inhibitor组穿膜细胞数量低于NC-inhibitor组(P<0.05);细胞划痕实验显示,miR-196a mimic组细胞迁移距离高于NC-mimic组,miR-196a inhibitor组细胞迁移距离低于NC-inhibitor组(P<0.05)。Western blot实验结果显示,
To explore the prognostic value of miR-196a in esophageal carcinoma and its regulatory mechanism of biological behavior. Methods Esophageal carcinoma tissues and paracancerous tissues were collected from 120 patients. miR-196a expression level was detected by RT-PCR. The relationships between miR-196a expression and clinical data were analyzed. The esophageal carcinoma patients were followed up. The overallsurvival （OS） and disease-free survival （DFS） were recorded. Taking OS and DFS as evaluation indexes, the prognostic factors were evaluated by univariate and multivariate Cox proportional hazards models. The TE1 cells were transfected with miR-196a mimic, NC-mimic, miR-196a inhibitor and NC-inhibitor. Cell proliferative ability was detected by MTT assay, invasive ability was detected by Transwell assay, migratory ability was detected by cell scratch assay. ANXA1, NTN4, HMGA2 and HOXB8 protein expressions in the cells were detected by Western blot. Results The expression of miR-196a in the esophageal carcinoma tissues was significantly higher than that in the paracancerous tissues （p 〈 0.05）. In the TE1 cells, the expression of miR-196a in the miR-196a mimic group was significantly higher than that in the NC-mimic group, the expression of miR-196a in the miR-196a inhibitor group was significantly lower than that in the NC -inhibitor group （ p〈 0.05）, which confirmed the cell transfection experiment was successful. The esophageal carcinoma patients were divided into miR-196a high-expression group （51 cases） and miR-196a low-expression group （69 cases） according to the miR-196a expression. miR-196a expression was not related to age, sex, differentiation, N stage or tumor location （p 〉 0.05）. With the increase of T staging, TNM stage and tumor diameter, the high-expression rate of miR-196a was significantly increased （p 〈 0.05）. Survival analysis showed that OS and DFS in the miR-196a high-expression group were significantly lower than those in the miR-196a low-expression gro
出处
《中国现代医学杂志》
CAS
北大核心
2017年第13期50-57,共8页
China Journal of Modern Medicine
关键词
微小RNA
食管癌
预后
增殖
侵袭
迁移
microRNA
esophageal carcinoma
prognosis
proliferation
invasion
migration
