摘要
目的通过构建大风藤Cocculus orbiculatus根与茎的指纹图谱,对比两者相似度,为科学评价大风藤根与茎药用价值及临床应用提供参考。方法采用高效液相色谱法对贵州省14个产地20批的大风藤根与茎进行色谱分离,色谱条件为Agilent ODS C_(18)(250 mm×4.6 mm,5μm)色谱柱;流动相为乙腈-0.1%磷酸水溶液,梯度洗脱,运行时间为95 min,体积流量1.0 mL/min,测定波长254 nm,柱温30℃。使用"中药色谱指纹图谱相似度评价系统2.0"进行数据处理,对大风藤根与茎进行相似度分析。结果建立了大风藤根与茎指纹图谱共有模式,归纳出大风藤根与茎共有峰8个,指纹图谱相似度为0.985~0.998。结论大风藤根与茎化学成分相近,但不同产地和部位的大风藤所含活性物质的量具有一定差异。
Objective To evaluate the similarity of Cocculus orbiculatus root and stem by high performance liquid chromatography （HPLC） fingerprint, and to provide a reference for evaluation and clinical application of C. orbiculatus root and stem. Methods HPLC was applied to chromatographic separation of C. orbiculatus root and stem in different seasons from 14 areas of Guizhou province. The chromatography conditions were： Agilent ODS C18（250 mm×4.6 mm,5μm） column, mobile phase of CAN 0.1% H3PO4, running time of 95 min, flow speed of 1.0 mL/min, detection wavelength of 254 nm, and column temperature of 30℃. The data were processed by Chinese medicine chromatographic fingerprint similarity evaluation system 2.0. Different C. orbiculatus root and stem were subjected to similarity analysis. Results The chromatography fingerprint of C. orbiculatus root and stem was established and eight common characteristic peaks were demonstrated. The similarities of the chromatography fingerprint were between 0.985-0.998. Conclusion The chemical compositions ofC. orbiculatus root and stem is similar, but the active substances quantity of C. orbiculatus from different regions and seasons are different.
出处
《中草药》
CAS
CSCD
北大核心
2017年第12期2522-2526,共5页
Chinese Traditional and Herbal Drugs
关键词
大风藤
根
茎
相似度
高效液相色谱
指纹图谱
Cocculus orbiculatus （L.） DC.
root
stem
similarity
HPLC
fingerprint
