摘要
通过建立团羽铁线蕨指纹图谱和多种共有成分含量测定方法,以在不同产地团羽铁线蕨质量控制中应用。采用SHISEIDO CAPCELL PAK C18色谱柱(4.6 mm×250 mm,5μm),检测波长280 nm,检测温度35℃,甲醇-0.05%磷酸水溶液梯度洗脱方法建立铁线蕨指纹图谱与特征成分含量测定的HPLC-DAD分析方法,对多批次药材以相似度软件与化学计量学分析相结合的方式进行质量评价。结果表明:本方法提取铁线蕨有13个共有峰,指认出共有峰2个,分别为槲皮素-3-O-β-D-吡喃葡糖苷酸和山奈酚-3-O-芸香糖苷,相似度为0.558~0.901。结合聚类分析和主成分分析,使用化学计量学正交偏最小二乘判别分析法对13个共有峰进行评价,样本中槲皮素-3-O-β-D-吡喃葡糖苷酸和山奈酚-3-O-芸香糖苷含量范围分别为0.11~4.35 mg/g、0.15~10.35 mg/g,结果显示不同产地团羽铁线蕨数据差异大。建立的指纹图谱及多特征成分含量测定的HPLC-DAD分析方法,能更全面而准确地体现团羽铁线蕨药化学成分整体轮廓与各类特征成分含量贡献,为提升不同产地团羽铁线蕨药材的质量控制方法奠定了基础。
The method of fingerprinting and content determination of multi-characteristic components of Adiantum capillus-junonis Rupr.was established and applied to the quality control of A.capillus-junonis Rupr.in different origin.Method:The chromatographic condition was set up on the SHISEIDO CAPCELL PAK C18 chromatographic column(4.6 mm×250 mm,5μm)by gradient elution of methanol-0.05%phosphoric acid,the monitoring wavelength was 280 nm,detection temperature was 35℃.The technique was used to establish a HPLC-DAD analysis method for the analysis of fingerprint and multi-characteristic components of A.capillus-junonis Rupr.And similarity software and chemometrics analysis was used to quality evaluation of multi-batch medicinal materials.There were 13 common peaks in this method,and 2 were identified as quercetin-3-O-β-D-glucuronide and kaempferol 3-O-rutinoside respectively,similarity ranged from 0.558 to 0.901,combined with cluster analysis and principal component analysis,13 common peaks were evaluated by orthogonal partial least squares discriminant analysis,and the contents of quercetin-3-O-β-D-glucuronide and kaempferol 3-O-rutinoside were determined in the range of 0.11~4.35 mg/g and 0.15~10.35 mg/g,respectively.It can be seen from the results that there is a great difference in the A.capillus-junonis Rupr.medicinal materials from different habitats.The HPLC-DAD analysis method for fingerprinting and content determination of multi-characteristic components of A.capillus-junonis Rupr.can more comprehensively and accurately reflect the contribution of the overall chemical composition of A.capillus-junonis Rupr.and the content of various characteristic components.It lays a foundation for improving the quality control method of A.capillus-junonis Rupr.
作者
王宇
林肯
肖丹
高源
Wang Yu;Lin Ken;Xiao Dan;Gao Yuan(Institute of Plant Protection,Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou 550006,China;Xinzhai Town Health Center,Yinjiang County,Tongren 555200;Guizhou institute of food inspection,Guiyang,Guizhou 550004,China)
出处
《山地农业生物学报》
2022年第5期8-14,共7页
Journal of Mountain Agriculture and Biology
基金
贵州省农业科学院青年科技创新工程项目(黔农科院青年基金[2022]32)
贵州省科技厅科技支撑计划项目(黔科合支撑[2017]2841)
贵阳市现代药业计划项目(筑科合同2018-11-3)。
关键词
团羽铁线蕨
HPLC-DAD指纹图谱
含量测定
方法学考察
Adiantum capillus-junonis Rupr.
HPLC-DAD fingerprint
content determination
methodological examination
