摘要
目的观察三七总皂苷(Panax notoginseng saponins,PNS)对TNF-α诱导的小鼠肾小球系膜细胞SV40 MES 13增殖及基质金属蛋白酶/基质金属蛋白酶组织抑制物(MMPs/TIMPs)表达的影响。方法采用TNF-α诱导SV40 MES 13细胞增殖,x CELLigence RTCA DP实时细胞分析系统记录120 h细胞指数(cell index,CI),实时荧光定量PCR法检测96 h SV40MES 13细胞MMP-2、TIMP-2m RNA表达,ELISA法检测96 h SV40 MES 13细胞上清MMP-9、TIMP-1表达。结果 PNS3个剂量组均能抑制TNF-α诱导的SV40 MES 13增殖,其中96 h效果最明显;PNS高、中剂量组能提高MMP-2/TIMP-2比值和MMP-9/TIMP-1比值,与TNF-α组比较差异显著(P<0.05)。结论 PNS能减轻TNF-α诱导的系膜细胞增殖,其机制可能与改善MMP-2/TIMP-2、MMP-9/TIMP-1平衡有关。
OBJECTIVE To study the effect of Panax notoginseng saponins（PNS） on TNF-α-induced proliferation of Mesangial cells and expression of matrix metalloproteinases（MMPs） and tissue inhibitors of metalloproteinases（TIMPs）. METHODS Proliferation of SV40 MES 13 cells was induced by TNF-α. Real-time cell analysis system was used to record 120 h cell index（CI）. The m RNA level of MMP-2 and TIMP-2 were measured by q RT-PCR. The expression of MMP-9 and TIMP-1 were measured by ELISA. RESULTS All three dose groups of PNS could inhibit the cell proliferation induced by TNF-α, of which 96 h effect was obvious. High, medium dose groups could significantly increase the ratio of MMP-2/TIMP-2 and MMP-9/TIMP-1 compared with TNF-α group（P〈0.05）. CONCLUSION PNS can inhibit the proliferation of Mesangial cells induced by TNF-α, and the mechanism may be related to the improvement of MMP-2/TIMP-2, MMP-9/TIMP-1 balance.
作者
童晔玲
任泽明
陈璇
戴关海
朱婉萍
TONG Yeling KEN Zeming CHEN Xuan DAI Guanhai ZHU Wanping(Zhejiang Institute of Traditional Chinese Medicine, Hangzhou 310007, China)
出处
《中国现代应用药学》
CAS
CSCD
2017年第6期820-823,共4页
Chinese Journal of Modern Applied Pharmacy
基金
浙江省科技厅项目(2015F10015)
