摘要
目的:积雪草颗粒对TGF-β1诱导的体外培养的肾小管上皮细胞MMP-2、TIMP-2 mRNA及蛋白表达的影响。方法:将体外培养的大鼠近端肾小管上皮细胞(NRK52E)随机分为6组:正常对照组(DZ组)、TGF-β1诱导组(T组)、积雪草小(JX组)、中(JZ组)、大剂量(JD组)组及蒙诺组(M组)。培养48 h后取出,应用实时荧光定量PCR(RTFQ PCR)技术和western-blot技术检测细胞MMP-2、TIMP-2的mRNA及蛋白表达。结果:与DZ相比,T、JX、JZ、JD、M组细胞MMP-2、TIMP-2的mRNA及蛋白表达明显升高(P<0.05);JX、JZ、JD、M组细胞MMP-2、TIMP-2的mRNA及蛋白与T组相比明显下降(P<0.05),M组细胞变化与JD组相似(P>0.05)。结论:积雪草抗TIF作用可能通过抑制MMP-2、TIMP-2的高表达,调节MMP-2/TIMP-2的平衡而实现,且与其剂量呈正相关。
Objective：We investigated Centella asiatica particles on TGF-β1 -ind-uced in vitro cultured tubular pithelial cells of the mRNA and protein of MMP-2,TIMP-2 expression. Methods：Rat proximal tubular epithelial cells （NRK52E） cultured in vitro were randomly divided into 6 groups：Normal control group（group N）,TGF-β1 -induced （10 ng/ml） group（group T）,low （ group JX） , medium （ group JZ ） , hi - gh （ group JD ） Centella asiatica group, Monopril group （ group M ） . Take these cells after 48 hours,and application of realtime fluorescence quantitative PCR（RTFQ PCR） to detect the mRNA of MMP-2and TIMP-2,appli-cation of western-blot detect their protein. Results：Compared with the group DZ,the expression of MMP-2,TIMP-2 mRNA and protein in group T,JX,JZ,JD and M was significantly increased （P0. 05）. Conclusion：The centella asiatica in the role of anti-TIF may be provided by inhibiting the expression of MMP-2,TIMP-2 and adjusting the balance of MMP-2/TIMP-2,and positively correlated with their dose.
出处
《中国中西医结合肾病杂志》
2014年第5期389-391,共3页
Chinese Journal of Integrated Traditional and Western Nephrology
基金
国家自然基金资助项目(No.81173409)
