摘要
目的：探讨过氧化物酶体增殖物激活受体（PPAR）γ是否参与单磷酸腺苷激活的蛋白激酶（AMPK）激活对心肌肥厚调节。方法：采用[3H]亮氯酸掺入、RT-PCR和Western blot等实验方法，观察AMPK激活与抑制时对笨肾上腺素（PE）诱导的培养新生SD大鼠心肌细胞肥大的影响及PPARγ的mRNA与蛋白表述的变化。结果：AMPK激活或抑制可抑制或增加PE诱导心肌细胞蛋白合成，同时伴随有PPARγ mRNA及蛋白表达的增减。结论：PPARγ参与了AMPK激活对心肌肥厚的调节。
Objective ：To investigate that peroxisome proliferator activated Teceptor （PPAR） gamma is involved in the regulation of cardiac hypertrophy by activation of adenosine monophosphate activated protein kinase （AMPK）.Method： To understand the effect of hypertrophy and expressions of mRNA and protein of PPAR gamma in the cultured myocardial cells of newborn SD rats induced by phenylephrine （PE）and AMPK activation, through [3H]-leucine incorporation, RT-PCR and Western blot.Results： AMPK activation or inhibition can inhibit or increase myocardial cell protein synthesis induced by PE, accompanied by the increase of mRNA and protein expression of PPAR gamma. Conclusion： PPAR γ is involved in the regulation of cardiac hypertrophy by AMPK activation.
出处
《国际心血管病杂志》
2017年第A01期232-233,共2页
International Journal of Cardiovascular Disease
