摘要
目的在原代培养的新生大鼠心肌细胞上，观察红景天苷（sal）对苯肾上腺素（PE）诱导的心肌细胞肥大的影响。方法应用细胞培养技术培养新生大鼠心肌细胞，采用倒置显微镜和图像分析测量细胞面积，测定心肌细胞。H．亮氨酸掺入率作为心肌细胞肥大的指标；用Western blot法检测心肌细胞热休克蛋HSP70蛋白的表达。结果浓度50Hg／ml的sal能抑制PE诱导的心肌细胞增大及心肌细胞合成速率的增加（P〈0．05）；能显著增加心肌肥大细胞HSP70蛋白表达（P〈0．05）。结论sal可以抑制PE诱导的心肌细胞肥大，这可能与其增加HsP70蛋白表达有关。
Objective To explore the regulatory effect of salidroside （ Sal ） on HSP70 expression in improving rat myocardial hypertrophy. Methods Cultured cardiomyocytes Of nude rats were used to establish a myocardial hypertrophy model by phenylephrine （ PE ） stimulation. The cardiomyocyte surface area was detected by inverted microscope and digital image analysis technology. 3H-leucine incorporation was measured to evaluate myocardial hypertrophy. The expression of HSP70 protein was detected by Western blot. Results Sal （ 50p.g/ml ） was able to decrease the increased cell surface area and the 3H-leucine incorporation was stimulated by PE （ P〈0.05 ） . Sal was able to increase HSP70 protein expression. Conclusion Sal can ameliorate myocardial hypertrophy, probably through increases HSP70 protein expression.
出处
《浙江临床医学》
2017年第7期1213-1215,共3页
Zhejiang Clinical Medical Journal
