摘要
目的:探讨EB病毒编码的BARF1基因对鼻咽癌细胞JNK/c-Jun信号通路活性及bcl-2表达的影响。方法:以p SG5空载体转染鼻咽癌细胞系CNE1(CNE1-SG)为对照,采用蛋白质印迹法检测稳定表达BARF1的CNE1细胞(CNE1-BARF1)中JNK/c-Jun通路的活性及BCL-2的表达;经JNK特异性抑制剂SP600125处理后,检测CNE1-BARF1细胞中JNK/c-Jun通路的活性及BCL-2的表达。结果:与CNE1-SG细胞相比,CNE1-BARF1细胞中cJun、BCL-2表达以及JNK和c-Jun蛋白的磷酸化水平明显增加(P<0.05)。经SP600125处理后,CNE1-BARF1细胞中c-Jun及BCL-2表达明显降低(P<0.05),c-Jun磷酸化水平也降低,但差异无统计学意义(P>0.05)。结论:BARF1基因可能通过激活JNK/c-Jun信号通路上调原癌基因bcl-2的表达。
Objective：To investigate the effect of Epstein-Barr virus encoded BARF1 gene on the activation of JNK/c-Jun signal pathway and bcl-2 expression in human nasopharyngeal carcinoma cells.Methods：Western blotting was applied to analyze the activation of JNK/c-Jun pathway and the expression of BCL-2 in BARF1-stably-expressing human nasopharyngeal carcinoma cell line CNE1 （CNE1-BARF1） and the control of CNE1 cell transfected by pSG5 vector（CNE1-SG）.After blocked by the JNK special inhibitor SP600125, the activation of JNK/c-Jun and the expression of BCL-2 were also analyzed in CNE1-BARF1 cells.Results：Compared with the control CNE1-SG, the level of c-Jun and BCL-2 protein expression, and phosphorolization of JNK and c-Jun protein were significantly increased in CNE1-BARF1（P〈0.05）.The expression of c-Jun and BCL-2 protein were decreased after treated by inhibitor SP600125（P〈0.05）, and the level of c-Jun protein phosphorylation also decreased, but did not reach statistical significance（P〉0.05）.Conclusion：BARF1 could upregulate protooncogene bcl-2 expression through activating JNK/c-Jun signal pathway in human nasopharyngeal carcinoma cells.
出处
《江苏大学学报（医学版）》
CAS
2017年第3期209-212,共4页
Journal of Jiangsu University：Medicine Edition
基金
江苏大学高级技术人才科研启动基金资助项目(06JDG011)
