摘要
目的探究母代营养过剩对雄性子代能量代谢的影响。方法 C57BL/6J雌鼠随机分为高脂饮食组和正常饮食组,分别饲喂高脂或正常饲料2个月,然后与正常饮食C57BL/6J雄鼠交配,孕鼠在孕期和哺乳期继续摄取高脂或正常饲料。子一代雄鼠性成熟后与正常饮食C57BL/6J雌鼠交配得到子二代,子二代雄鼠性成熟后再与正常饮食C57BL/6J雌鼠交配得到子三代。测定子一代、子二代、子三代雄性小鼠体重、摄食量及随机血糖值。对子一代和子二代成年雄性小鼠进行腹腔糖耐量实验并记录肝脏、附睾周脂肪、皮下脂肪湿重值。检测子二代成年雄性小鼠血清胰岛素及下丘脑能量代谢相关基因表达情况。结果营养过剩母鼠子一代和子二代雄性小鼠的体重、摄食量、能量摄入量及随机血糖值均高于对照组(均P<0.05),子三代之间的差异则无统计学意义(P>0.05)。营养过剩母鼠子一代和子二代的成年雄性小鼠的附睾周脂肪脂体比、皮下脂肪脂体比、肝体比及糖耐量曲线下面积均高于对照组(均P<0.05)。营养过剩母鼠子二代成年雄性小鼠的血清胰岛素浓度及下丘脑神经肽Y(NPY)、刺鼠相关蛋白(AGRP)、甘丙肽(GAL)、可卡因-安他非明转录调节肽(CART)、SIM1基因的表达量均高于对照组(均P<0.05)。结论高脂饮食诱导的营养过剩母代对雄性子代能量代谢的影响可持续至子二代,引起子二代下丘脑能量代谢相关基因表达异常。
Objective To explore the effect of maternal nutrient excess on the energy metabolism of male offspring.Methods C57BL/6Jfemale mice were randomly divided into high fat diet group and normal diet group.They were fed with high fat or normal diet for 2months and then mated with C57BL/6Jmale mice fed on normal diet.Pregnant mice continued to ingest high fat or normal diet during pregnancy and lactation.After sexual maturation,the male mice of first generation were mated with C57BL/6Jfemale mice fed on normal diet.The mature male mice of the second generation were then mated with C57BL/6Jfemale mice fed on normal diet to obtain the third generation.The body weight,food intake and random blood glucose level were measured in the male mice of the first,second and third generation.Abdominal glucose tolerance test was performed on the adult male mice of the first and second generation.The wet weight of liver,epididymal fat and subcutaneous fat was also recorded.The levels of serum insulin and expression of hypothalamic energy metabolism related genes were detected in the adult male mice of the second generation.Results The body weight,food intake,energy intake and randomblood glucose levels of the mice of the first and second generation with nutritional excess mothers were significantly higher than those of the control group（all P〈0.05）,and the differences between the third generation mice and the control group were not statistically significant（P〈0.05）.Compared with the control group,the body-fat ratio of epididymal fat and subcutaneous fat,hepatosomatic index and area under the curve of glucose tolerance were higher in the first and second generation mice with nutritional excess mothers（all P〈0.05）.The serum insulin level and expression of Neuropeptide Y（NPY）,Agouti-Related Peptide（AGRP）,Galanin（GAL）,Cocaine-and Amphetamine-Regulated Transcript（CART）and Single-minded 1（SIM1）in the second generation male mice with nutritional excess mothers were significantly higher than those in the c
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2017年第3期258-264,共7页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
华中科技大学青年教师基金资助项目(No.2015QN110)
关键词
营养过剩
能量代谢
子代
能量代谢相关基因
nutrient excess
energy metabolism
offspring
energy metabolism related genes
