摘要
近年来多环芳烃(PAHs)衍生物因其潜在的毒性和环境中的频繁检出受到越来越多的关注.许多PAHs衍生物具有比母体化合物更高的极性,易迁移至地下,对地下水安全构成潜在威胁.PAHs及其衍生物在地下水中共存,具有含量低、种类多、性质差异大的特点,实现同时分析测定较为困难.本文通过液液萃取和固相萃取两种前处理方式的对比和色谱条件的优化,确定了采用液液萃取-超高效液相色谱同时测定地下水中16种PAHs母体化合物、3种含氮衍生物、1种含氯衍生物的分析方法.超高效液相色谱选用ACQUITY UPLC BEH Shield RP18色谱柱,以乙腈和纯水作为流动相,在15.0 min内即可完成20种目标化合物的基线分离与分析,检测效率比高效液相色谱分析提高了近一倍.方法在5个浓度范围内均具有良好的线性关系(R2>0.9990).除苊烯外,其他PAHs化合物的方法检出限均在0.02—1.50 ng·L^(-1)之间.20种目标化合物的加标回收率在60%—103%之间.本方法成功地应用于实际地下水样品的分析,对批量地下水中PAHs及衍生物的同时筛查分析具有较强的实用意义.
Many polycyclic aromatic hydrocarbons（PAHs） derivatives have higher toxicity than their parent compounds,and are prone to leaching into groundwater,which pose potential threats to human health. It is difficult to simultaneously determinate PAHs and their derivatives in groundwater due to their low concentrations and different properties. The existing analytical methods mainly aim atthe 16 priority parent compounds and few reports looked into simultaneous determination of PAHs and their derivatives. In this work,a method was developed for the simultaneous determination of 16 PAHs,3 nitrogen containing derivatives and 1 chlorinated derivative in groundwater by ultra-high performance liquid chromatography. Two pretreatment methods,LLE and SPE were compared,and the optimization of the UHPLC condition was carried out. The chromatographic separation was carried out on ACQUITY UPLC BHE Shield RP 18 column. Complete seperation of 20 analytes was achieved in 15 min. The linearity range was from 0.5 to 1000 ng·mL^-1with good correlation coefficient（R20.999）. The recoveries in groundwater were between 60%—103%. Except for acenaphthylene,the MLD ranged from 0.02 to 1.50 ng·L^-1. This work provided an alternative method to screen PAHs and their derivatives in groundwater,which is of great practical significance.
出处
《环境化学》
CAS
CSCD
北大核心
2017年第6期1295-1303,共9页
Environmental Chemistry
基金
国家自然科学基金青年科学基金(21507017)
中央级公益性科研院所基本科研业务费项目(yywf201522)
中国地质调查局地质调查项目(DD20160312)
中国科学院生态环境研究中心环境化学与生态毒理学国家重点实验室开放基金(KF2016-06)资助~~
关键词
地下水
多环芳烃
衍生物
液液萃取
超高效液相色谱法
groundwater
polycyclic aromatic hydrocarbons（PAHs）
derivatives
liquid-liquid extraction
Ultra-high performance liquid chromatography
