摘要
建立同时测定肉类食品中尿酸、鸟嘌呤、次黄嘌呤、黄嘌呤和腺嘌呤的高效液相色谱分析方法,并测定肉类样品中上述4种嘌呤及尿酸的含量。色谱条件:资生堂CAPCELL PAK-C_(18)(4.6 mm×250 mm,5μm)色谱柱,柱温30℃;流动相为7×10^(-3)mol/L KH_2PO_4-H_3PO_4(pH=3.83),流速1.0 mL/min;检测器为紫外检测器,检测波长254 nm;进样量10μL。结果:在上述条件下4种嘌呤和尿酸分离和测定效果良好,4种嘌呤和尿酸的质量浓度和峰面积在0.05~50μg/mL线性范围内线性关系良好,相关系数均在0.999 6以上,检出限在0.010~0.024μg/mL之间,精密度检测RSD为0.25%~0.81%,各组分回收率为92.0%~105.0%,方法精密度RSD为6.27%~11.09%。结论:该方法简便,快捷,可靠,各组分分离度好,可应用于肉类食品中嘌呤和尿酸的同时分析测定。
A high performance liquid Chromatography method for the determination of uric acid, guanine, hypo- xanthine, xanthine and adenine in meat was established and applied. The detecting method for Chromatography was： Shiseido CAPCELL PAK-C1s （4.6 mm × 250 mm, 5 μm）column, column temperature was at 30℃. The mobile phase was 7 × 10-3 mol/L KH2PO4-H3PO4 （pH = 3.83 ）at a flow rate of 1.0 mL/min. The UV detector was used and the wavelength was set at 254 nm and the Sample volume was 10μL. Result： Under the above conditions, good separa- tion was obtained and four kinds of purines and uric acid was showed in the range of 0.05 -50 μg/mL; a good linear relation was observed between the concentration and peak area,and all the correlation coefficients are higher than 0. 9996, the limit of detection was between 0. 010 -0. 024 g/mL, precision detection of RSD% was between 0.25% - 0.81% ,the recovery rate of each component was between 92.0% -105.0% , the precision RSD% was between6. 27% -11.09%. Conclusion： The method was simple, rapid and reliable,had a good separation for each component, and can be used for the analysis and determination of purine and uric acid in meat products.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2017年第4期232-237,共6页
Food and Fermentation Industries
基金
基金项目:青藏高原特色风味食品中危害物质高通量筛查及快速检测技术研究(2014-ZJ-722)
