摘要
槐糖脂是酵母菌产生的一种重要的生物表面活性剂,具有抗肿瘤活性.纳米羟基磷灰石(Nano-hydroxyapatite,n HA)因具有良好的生物活性及生物相容性而被广泛应用于临床载体,与药物相负载.通过n HA与内酯型双乙酰槐糖脂(Lactonic diacetyl-sophorolipids,L-SLs)最适搭载率的确定及负载后药物缓释能力的测试、细胞毒性研究、细胞形态观察,探讨搭载后的L-SLs对人宫颈癌Hela细胞的抑制作用.结果显示:最适搭载率为1:5,且L-SLs浓度为5.0 mg/m L时负载率可以达到最大值99.47%,负载后药物缓释时间为14 h,L-SLs对人宫颈癌Hela细胞的增殖有良好的抑制作用,并且细胞形态发生了明显的变化,细胞膜鼓出,体积减小,细胞肿胀裂解成碎片、细胞解体.本研究表明,L-SLs能够抑制人宫颈癌Hela细胞的增殖,n HA搭载L-SLs后能够延长槐糖脂的释放时间.
Sophorolipids （SLs）, important extracellular biosurfactants produced by yeasts, have anticancer activity. Nano-hydroxyapatite （nHA） loaded with therapeutic agents is widely applied in the pharmaceutical industry for its biological activity and compatibility. Therefore, it is important to explore the pharmaceutical activity of SLs and nHA-SLs on cervical cancer cells. The effects of lactonic diacetyl-sophorolipids （L-SLs） on cancer cells were studied by determining the optimal load rate, conducting a slow-release ability test, analyzing cell toxicity, and observing cell morphologies. The results showed that the optimal load rate was 1：5 for nHA and L-SLs, slow-release was approximately 14 h, with 99.47% of L-SLs loaded at a concentration of 5.0 mg/mL, HeLa cell growth was inhibited, and cell morphological changes were observed, including cell swelling, cell size decrease, and cell destruction and collapse. These data showed that SLs exert anticancer activity against HeLa cells and that the slow-release ability of L-SLs is enhanced by loading on nHA.
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2017年第3期486-490,共5页
Chinese Journal of Applied and Environmental Biology
基金
齐鲁工业大学博士基金资助~~
