摘要
目的合成药物载体纳米羟基磷灰石(nano-hydroxyapatite,nHAP),考察其对于模型药物阿霉素的载药性能。方法采用化学共沉淀法,以Ca(NO3)2·4H2O和(NH4)2HPO4分别为钙、磷源,用氨水调控溶液p H值合成nHAP,考察温度、反应时间和陈化时间对于nHAP合成的影响。采用X射线衍射仪、扫描电镜、红外光谱仪、动态光散射粒度测定仪对于产品的晶相、晶貌、化学组成和粒径进行表征。采用溶液吸附法考察nHAP用量、溶液质量浓度和p H值对于nHAP负载阿霉素的影响。结果当反应温度60℃、反应时间2 h、陈化时间16 h时,所制备的nHAP为长(138±35)nm,宽(39±8)nm棒状纳米粒,晶体形貌规则,结晶良好。负载阿霉素的研究表明,所合成的nHAP载药性能良好,载药量达到42.43 mg·g^(-1)。结论 nHAP对于阿霉素具有良好的承载能力,可作为阿霉素的优良载体。
Objective To synthesis nano-hydroxyapatite （nHAP）as drug carder and investigate the drug loading properties for doxorubicin. Methods Chemical co-precipitation method was used to synthesis nHAP with [ Ca （ NO3 ） 2· 4H2O ] and [ （ NH4 ） 2 HPO4 ] as the sources of calcium and phosphorus, respectively. The pH of reactive system was controlled by addition of ammonia. Factors affecting the formation of nHAP crystals were evaluated, such as temperature, reaction time, aging time. The crystal phase, crystal morphology, chemical composition, particle size of the obtained crystals were characterized by using X-ray diffraction, scanning electron microscopy, infrared spectrometry and laser particle size tester. Doxorubicin （DOX·HCl） was loaded to nHAP by solution absorption method, and the influences of nHAP amount, concentration of DOX·HCl and pH value on the drug loading were investigated. Results The sample of nHAP obtained at the optimum process condition with temperature at 60 ℃, reaction time of 2 h, aging time of 16 h, was rod-like with a length of （138 ＋ 35 ）nm and a width of （39 ＋ 8 ）nm. Meanwhile, the crystals had uniform morphology and fine crystallinity. Synthesized nHAP was shown to have a good capacity for adsorbing DOX. HC1 with the drug loading of 42.43 mg·g^-1.. Conclusions nHAP can be used as a potential drug carrier.
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2017年第1期1-6,22,共7页
Journal of Shenyang Pharmaceutical University
基金
辽宁省教育厅科学技术研究项目(L2014395)
沈阳药科大学国家级大学生创新创业训练计划项目(201510163025)
关键词
纳米羟基磷灰石
化学共沉淀
阿霉素
载药
nano-hydroxyapatite
chemical co-precipitation
doxorubicin
drug loading
