摘要
目的:观察电针对局灶脑缺血/再灌注大鼠大脑缺血皮质区白介素-4(IL-4)含量,信号传导及转录激活因子6(STAT 6)、磷酸化STAT 6(pSTAT 6)与核因子-κB(NF-κB)p 65蛋白表达的影响,探讨电针治疗局灶脑缺血/再灌注后炎性反应的可能机制。方法:SD大鼠随机分为假手术组、模型组、电针组,每组15只。采用改良线栓法制备大脑中动脉闭塞/再灌注模型。电针组电针刺激大鼠"百会""合谷""太冲",再灌注0、12、24h各电针1次。运用免疫组化及Western blot法检测大鼠大脑缺血皮质区pSTAT 6/STAT 6、NF-κB p 65的表达水平;ELISA法检测大鼠大脑缺血皮质区IL-4的含量。结果:与假手术组比较,模型组大鼠大脑缺血皮质区IL-4含量显著升高(P<0.001),胞质及胞核NF-κB p 65表达水平显著增加(免疫组化法P<0.001,Western blot法P<0.01);与模型组比较,电针组IL-4含量显著升高(P<0.01),pSTAT 6及pSTAT 6/STAT 6表达水平显著升高(均P<0.01),胞质及胞核NF-κB p 65表达水平显著降低(免疫组化法P<0.01,Western blot法P<0.05)。结论:电针能有效提高局灶脑缺血/再灌注损伤大鼠大脑缺血皮质区抗炎因子IL-4含量,增加pSTAT 6/STAT 6表达水平,同时NF-κB p 65入核显著减少,可能与激活IL-4/STAT 6信号通路有关。
Objective To observe the effect of electroacupuncture （EA） on expression of cerebral cortical interleukin 4 （IL-4）, signal transducer and activator of transcription 6 （STAT 6） and NF-κB family subunit p 65 in focal cerebral ischemia/reperfusion injury （CIRI） rats, so as to explore its mechanisms in reducing cerebral ischemia. Methods Forty-five male SD rats were randomly divided into sham group （n = 15）, CIRI model group （n = 15）, and EA group （n = 15） . The CIRI model was established by middle cerebral artery occlusion/reperfusion （MCAO/R）. EA （1 mA, 2 Hz/100 Hz） was applied to ＂Baihui＂（GV 20） and the left ＂Hegu＂ （LI 4）-＂Taichong＂ （LR 3） for 20 min, once every 12 h, three times altogether. The expression levels of p-STAT 6, STAT 6 and NF-κB p 65 in the ischemic cortex were detected by immunohistochemistry and Western blot, separately, and the content of IL-4 was detected by ELISA. Results Compared with the sham group, cerebral IL-4 content and the expression levels of NF-κB p 65 immunoactivity and protein were significantly up-regulated in the CIRI model group （ P〈0. 001, P〈0. 01 ）. Following EA intervention, the levels of IL-4, p-STAT 6 expression and ratio of p-STAT 6/STAT 6 were notably increased in the EA group than in the model group （P〈0. 01）, while the levels of cerebral NF-κB p 65 immunoactivity and protein expression were significantly down-regulated （P-〈0.01, P〈0.05）. Conclusion EA of GV 20 and LI 4-LR 3 can up-regulate cerebral cortical IL-4 content and p-STAT 6 expression, and down-regulate NF-κB p 65 expression in CIRI rats, which may contribute to its effect in improving cerebral ischemia by reducing inflammatory injury.
出处
《针刺研究》
CAS
CSCD
北大核心
2017年第3期217-222,258,共7页
Acupuncture Research
基金
国家自然科学基金项目(No.30470606)
国家自然科学基金青年基金(No.81403243)
重庆市卫生局中医药科技项目(No.2012-2-128)
