摘要
目的:探讨针刺捻转泻法对应激性高血压前期(SI-PHT)大鼠的作用机制。方法:Wistar大鼠随机分为空白组(12只)、模型组(12只)和捻转泻法组(10只)。以足底电击结合噪声刺激方法复制SI-PHT模型。造模期间,每日对捻转泻法组大鼠双侧"太冲""曲池"行针刺捻转泻法操作。实验前1天,第3、5、7、9、11天测量各组大鼠收缩压,并观察行为学变化;以基因芯片技术检测并分析各组下丘脑基因表达谱。结果:与空白组相比,第3~11天模型组和捻转泻法组大鼠收缩压均明显升高(P<0.01);与模型组相比,第5~11天捻转泻法组大鼠收缩压下降(P<0.01);模型组和捻转泻法组大鼠同时伴躁狂等行为学改变,但捻转泻法组较模型组减轻。40条基因在模型组与空白组、捻转泻法组与模型组的比较中表达趋势均相反(P<0.05,|FC|≥1.5);其中,Acsm 3、Cgα、CryαB和Tshβ与高血压明显相关(P<0.05,|FC|≥1.5);捻转泻法通过激活Autoimmune thyroid disease(AITD)通路发挥降压效应。结论:针刺捻转泻法可引起SI-PHT大鼠下丘脑基因Acsm 3、Cgα、CryαB和Tshβ的激活,出现生物学及分子功能的变化,并通过激活AITD通路发挥降压效应。
Objective To observe the effect of twirling-reducing needling on changes of profile of differentially-expressed genes in the hypothalamus in rats with stress-induced prehypertension （SI-PHT）, so as to explore its mechanism underlying improvement of prehypertension. Methods Wistar male rats were randomly divided into blank control group （n = 12）, model group （n = 12） and acupuncture group （n = 10）. The SI-PHT model was established by planta-electric-shock stimulation （30 V, 5 s） plus noise stimulation （80-100 db, 50 ms）, twice daily, continuously for 11 days. Manual acupuncture stimulation （twirling-reducing needling） was applied to bilateral ＂Taichong＂ （LR 3） and ＂Quchi＂ （LI 11） for about 1 rain （60 cycles /min）, followed by retaining the needles for 20 min during modeling preparation, once daily for 11 days. The systolic blood pressure （SBP） and behavior changes were recorded before intervention and on the 3^rd, 5^th, 7^th, 9^th and 11^th day during modeling. The ex- pression （Fold change, ｜ FC｜≥1. 5） profile of genes in the hypothalamus was analyzed using Affymetrix GeneOhip Rat Gene 2.0 ST Array, followed by analyzing the differentially-expressed genes involving the related biological process, molecular function, and signaling pathways by using Gene Ontology （GO） and Kyoto Encyclopedia of Genes and Genomes （KEGG） , separately. Results After modeling, the systolic pressure levels were remarkably increased from the 3^rd day to the 11^th day in both model and acupuncture groups relatively to their respective baseline levels （P〈0.01）, and were significantly lower in the acupuncture group than in the model group （P〈0.01） from the 5^th day to the 11^th day. Meanwhile, 93 genes were up-regulated and 153 down-regulated in the model group relative to the control group （ P〈0.05, ｜ FC｜≥1. 5）, while in comparison with the model group, 59 genes were up-regulated and 86 down-regulated in the acupuncture group （P〈0.05,｜ FC｜�
出处
《针刺研究》
CAS
CSCD
北大核心
2017年第3期209-216,共8页
Acupuncture Research
基金
国家自然科学基金项目(No.81373727)
关键词
针刺
捻转泻法
应激性高血压前期
下丘脑
转录谱
基因芯片
Acupuncture
Twirling-reducing needling
Stress-induced prehypertension
Hypothalamus
Transcriptional profiling
Gene chip
