摘要
目的建立微波消解-电感耦合等离子质谱法(microwave digestion-inductively coupled plasma mass spectrometry,MWD-ICP-MS)同时测定蜂胶中铅(Pb)、砷(As)、汞(Hg)、镉(Cd)、铬(Cr)5种常见重金属元素。方法样品加HNO_3 5.0 m L和H_2O_2 1.0 m L进行微波消解,赶酸温度为110℃;采用ICP-MS法直接测定Pb、As、Hg、Cd、Cr 5种重金属元素的含量。结果 Pb、As、Cd、Cr在1~500μg/L线性范围内,Hg在1~5.0μg/L线性范围内都具有良好的线性关系,相关系数r>0.999,检出限为0.012~0.015 mg/kg,加标回收率为95.3%~101.8%。该方法测得蜂胶中的Pb含量为2.16 mg/kg、As含量为0.23 mg/kg、Hg含量为0.03 mg/kg、Cd含量为0.06 mg/kg、Cr含量为1.2 mg/kg。结论该方法具有良好的准确度和精密度,检出限低,适用于蜂胶中常见重金属元素的同时测定。
Objective To establish a method for simultaneous determination of 5 kinds of common heavy metal elements including Pb,As,Hg,Cd and Cr in propoils by microwave digestion-inductively coupled plasma mass spectrometry （MWD-ICP-MS）.Methods After microwave digestion with 5 mL nitric acid and 1 mL hydrogen peroxide and acid removing at 110 ℃,the content of Pb,As,Hg,Cd and Cr in samples were determined by ICP-MS directly.Results There were good linear relationships in the ranges of 1-500 μg/L for Pb,As,Cd and Cr,and 1-5.0 μtg/L for Hg,the linear correlation coefficients were more than 0.999,the limits of detection were 0.012-0.015 mg/kg,and the recoveries were 95.3-101.8%.The content of Pb of propolis measured by the established method was 2.16 mg/kg,As was 0.23 mg/kg,Hg was 0.03 mg/kg,Cd was 0.06 mg/kg and Cr was 1.2 mg/kg.Conclusion The established method has high accuracy,good precision and low detection limit,which is suitable for simultaneous determination of heavy metal elements in propolis.
出处
《食品安全质量检测学报》
CAS
2017年第4期1441-1444,共4页
Journal of Food Safety and Quality
基金
衢州市科技局2016年度指导性科技项目(2016031)~~
关键词
微波消解
电感耦合等离子体质谱法
蜂胶
重金属元素
microwave digestion
inductively coupled plasma mass spectrometry
propolis
heavy metal elements
