摘要
同步辐射X射线显微成像具有极高的空间分辨率、很好的穿透深度和优秀的能量分辨,因而在纳米分辨细胞成像领域具有巨大的应用潜力。然而,目前X射线显微技术多用于细胞结构成像,而和该技术相适合的特异性识别细胞内重要生物靶标的分子探针仍较缺乏。本工作利用同步辐射X射线良好的能量分辨特点,经体外化学催化反应成功制备了同步X射线可见的成像标签,成像分辨率达到30 nm。研究结果为进一步制备X射线敏感的分子探针,实现对细胞内生物分子的特异性识别和成像打下了良好的基础。
Background： With short wavelengths, long penetration depth and excellent energy resolution of X-rays, synchrotron-based X-ray microscopy （XRM） has demonstrated its great potential for nanoscale cellular imaging. However, despite of the high-quality sub-cellular morphological details provided by XRM, it lacks appropriate molecular probes capable of localizing cellular targets. Purpose： This study aims to perform the preparation of X-ray imaging tags. Methods： In vitro chemical catalytic reaction was introduced to get the X-ray imaging tags. Results： By exploiting the excellent energy resolution of X-rays, synchrotron-based X-ray imaging tags were prepared with the imaging resolution of 30 nm when observed by soft X-ray microscopy at Shanghai synchrotron radiation facility （SSRF）. Conclusion： The results indicate high potential to design X-ray sensitive molecular probes for cell imaging.
出处
《核技术》
CAS
CSCD
北大核心
2017年第6期16-20,共5页
Nuclear Techniques
基金
中国科学院重大科技基础设施开放研究项目
中国科学院青年创新促进会(No.2016236)
国家重点研发计划(No.2016YFA0400902)
国家自然科学基金(No.11675251、No.21390414)
上海市青年科技英才扬帆计划(No.17YF1423600)资助~~
