摘要
目的制定高效液相色谱-蒸发光散射检测法测定散结通胶囊中胆酸的含量。方法采用Kromasil 100-5 C_(18)(250 mm×4.6 mm,5μm)色谱柱,以甲醇-0.1%冰醋酸(75∶25)为流动相,流速1.0 m L/min,进样体积20μL;蒸发光散射检测器参数:漂移管温度40℃,载气(N_2)压力200 k Pa。结果胆酸进样量在1.368~13.68μg范围内,进样量的对数值与峰面积的对数值呈良好的线性关系(r=0.9999),平均加样回收率为100.26%(RSD=2.4%,n=6)。结论本方法简便准确,可用于散结通胶囊的质量控制。
Objective To establish an HPLC-ELSD method for determination of cholic acid in Sanjietong Capsules. Methods HPLC was performed on a Kromasil 100-5 C18 column （250 mmx4.6 mm, 5 μm）, and the mobile phase was methanol-0.1 % glacial acetic （75：25） at a flow rate of 1.0 mL/min. The sample volume was 20 μL. The parameters of ELSD were set as follows： the evaporation temperature was 40 ℃, and the carrier gas （N2） pressure was 200 kpa. Results A good linear correlation of logarithm of sample quantity with peak area was observed within the range of 1.368-13.680 μg for cholic acid （r=0.9999）. The average recovery was 100.26 %, with RSD of 2.4 % （n=6）. Conclusion The method is convenient and accurate, and can be used for the quality control of Sanjietong Capsules.
出处
《食品与药品》
CAS
2017年第3期202-204,共3页
Food and Drug
