摘要
目的肛瘘愈创洗液主要用于克罗恩病肛瘘的创面清洗,控制炎症,促进伤口愈合。文中主要对肛瘘愈创洗液的提取工艺进行研究。方法采用正交试验法对肛瘘愈创洗液的加水量、煎煮时间、煎煮次数、药液比4个因素进行考察,每个因素3个水平,以组方中药所含的活性成分丹参素钠含量作为评价指标,并以高效液相色谱法测定丹参素钠的含量,根据测定结果对肛瘘愈创洗液水提醇沉提取工艺进行优选。结果肛瘘愈创洗液的提取工艺经优选为:取丹参、千里光、黄芪、地榆四味中药加8倍量水浸泡过夜,煎煮3次,分别为2、1.5、1.5 h,合并煎液,滤过,滤液浓缩至药材与浓缩液之比为1∶1.5。工艺验试结果表明,3个批号丹参素钠含量分别为0.520、0.498、0.521 mg/mL,RSD分别为0.34%、0.41%、0.29%。结论优选的提取工艺合理可行,结果可靠,可用于肛瘘愈创洗液的生产制备。
Objective Ganglouyuchuang lotion was used to dress the wound for Anal fistula in Crohn＇s disease, control in- flammation and promote wound healing. This paper studied the extraction techonology of ganglouyuchuang lotion. Methods Four factors of ganglouyuchuang lotion, including volume of water, extraction time, extraction times and liquid ratio, were studied by the or- thogonal test, and three levels were selected for each factor. The content of sodium danshensu, the active component contained in Chi- nese herbal medicine, was regarded as evaluating indicator, and the content of Danshensu Sodium was determined by HPLC. The water extraction and alcohol precipitation technology of ganglouyuchuang lotion was optimized according to the results of measurement. Results The optimum extraction technology of ganglouyuchuang lotion was as follows ： four herbs, including Salvia mihiorrhiza, Radix Astragali, Radix sanguisorbae and Senecio were added with8 times amount of water overnight and decocted 3 times with 2 h, 1.5 h, and 1.5 h respectively, and then the extraction was concentrated to the ratio of herbs and concentrate of 1 ： 1.5. The results showed that the contents of Danshensu Sodium from the three examples were 0.520, 0.498, and 0.521 mg/mL, and the RSD were 0.34%, 0.41%, and 0.29%. Conclusion The optimum extraction technology is feasible and applicable for the preparation of ganglouyuchuang lotion.
出处
《医学研究生学报》
CAS
北大核心
2017年第6期645-647,共3页
Journal of Medical Postgraduates
关键词
肛瘘愈创洗液
水提醇沉法
正交试验
丹参素钠
Ganglouyuchuang lotion
Water extraction and alcohol precipitation method
Orthogonal test
Sodium danshensu
