摘要
目的通过构建雄性SD大鼠慢性非细菌性前列腺炎(CNP)模型研究白细胞介素-1β(IL-1β)的表达,探讨IL-1β在CNP中的可能发挥的作用。方法取3月龄雄性SD大鼠30只随机分为正常对照组和CNP模型组,每组15只。造模后通过HE染色观察两组大鼠前列腺组织炎症浸润情况,并应用Western blot法、免疫组化SABC法检测两组SD大鼠前列腺组织中IL-1β表达情况。结果 HE染色结果证实成功构建出SD大鼠慢性非细菌性前列腺炎模型,Western blot法、免疫组化法检测都显示CNP模型组IL-1β的表达水平均高于正常对照组,差异有统计学意义(P<0.05)。结论IL-1β在SD大鼠CNP模型中表达水平增高,在前列腺炎症的发生发展过程中起着重要作用。
Objective To explore the possible role of interleukin-1β （ IL-1β ） in chronic prostatitis by exploring the expression level of IL-1β in chronic nonbacterial prostatitis （CNP） SD rat model. Methods 30 three-month-old male SD rats were randomly divided into two groups, 15 for control-group, and 15 for CNP model-group. To assess the prostate tissue inflammation of two groups,hematoxylin-eosin（HE） staining was used. Then the expression of IL-1β of the two groups were detected by Western blot and strept avidin-biotin complex （SABC） immunohistochem- istry. Results The results of HE staining showed that the SD rat model of CNP was successfully constructed. West- ern blot and immunehistochemistry showed that the expression of IL-1β in CNP model-group were higher than that of control-group, the difference was statistically significant （ P 〈 0.05 ）. Conclusion The expression of IL-1β is el- evated in CNP SD rat model and IL-1β may play an important role in the pathogenesis of CNP.
出处
《安徽医科大学学报》
CAS
北大核心
2017年第6期843-846,共4页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:81401518
81370856
81470986)
国家临床重点专科(含中医专业)建设项目(2012)
