摘要
目的:探讨低强度体外冲击波(Li-ESW)对慢性前列腺炎模型大鼠的治疗作用及其最佳参数。方法:2019年4—8月取90只8周龄健康雄性SD大鼠,按随机数字表法分为对照组(C组)15只,为未建模的大鼠;模型组(M组)15只,为建模后未治疗的大鼠;不同能量密度冲击波治疗组(T组)60只,为建模后进行Li-ESW治疗的大鼠。T组采用聚焦式体外冲击波治疗仪,大鼠平卧位,探头置于前列腺解剖位置上方。治疗方案:频率3 Hz,脉冲次数500次,每周1次共4周。根据能量密度将T组分为T1(0.09 mJ/mm^2)、T2(0.20 mJ/mm^2)、T3(0.30 mJ/mm^2)、T4(0.40 mJ/mm^2)组,每组各15只。建模前各组使用von Frey纤维丝于大鼠阴囊处分别采用2、4、6 g压力测试,记录阳性反应次数,各组间差异无统计学意义(P>0.05)。测痛实验1周后麻醉下采用3%卡拉胶注射大鼠前列腺两侧叶,构建慢性前列腺炎大鼠模型。建模1周后各组大鼠再次进行von Frey测痛实验,评估建模效果。然后T组开始治疗。每次治疗前各组均进行von Frey测痛实验。于治疗1、2、4周测痛实验后每组处死5只大鼠,无菌条件下剥离前列腺两侧叶行病理检查,对5个随机视野下前列腺间质的炎症细胞进行计数,每0~50个细胞记为1分,以评估炎症严重程度。结果:建模1周后,M组和各T组阳性反应次数均显著多于C组(P<0.01),M组与各T组间差异无统计学意义(P>0.05)。治疗后各时间点T2组与M组相比阳性反应次数均减少(P<0.05);T1、T3、T4组分别仅在治疗3周后、3周和4周后、2周后阳性反应次数少于M组(P<0.05)。T2组治疗1周后阳性反应次数开始下降,至4周后降至最低,各时间点测痛实验结果差异均有统计学意义(P<0.05)。病理检查结果显示,与C组比较,M组前列腺间质有较多炎症细胞浸润,上皮细胞排列出现紊乱。M、T1、T2、T3、T4组各时间点病理评分分别为:治疗1周后8、7、4、6、9分;2周后8、5、3、4、7分;4周后7、3
Objective To explore the therapeutic effect of low-intensity extracorporeal shock wave(Li-ESW)in model rats with chronic prostatitis and its optimal parameters.Methods From April to August 2019,90 healthy male SD rats aged 8 weeks were randomly divided into control group(group C,n=15),model group(group M,n=15)and shock wave treatment group(group T,n=60),which were treated with Li-ESW after being modeled successfully.The rats in group T were supine and treated with focused Li-ESW,and the probe was placed above the anatomical position of the prostate.Treatment scheme was 3 Hz of frequency,500 pulses,and once a week for 4 weeks.Group T was divided into group T1(0.09 mJ/mm^2),T2(0.20 mJ/mm^2),T3(0.30 mJ/mm^2)and T4(0.40 mJ/mm^2)according to energy flux density,with 15 rats in each group.Before the establishment of the model,the rats in each group were tested with von Frey fiber of 2 g,4 g and 6 g at the scrotum respectively,and the positive reaction was recorded.There was no significant difference between the three groups(P>0.05).After one week,the rat model with CP was established by injecting 3%carrageenan into the bilateral lobes of the prostate under anesthesia.And one week after the modeling was completed,the rats in each group underwent von Frey test again to measure pain and evaluate the modeling effects.Group T was then treated once a week for a 4-week period.During the treatment,von Frey test was performed before each treatment to evaluate the treatment effect of the previous week.After the von Frey test for one,two and four weeks of treatment,5 rats were sacrificed in each group,and the bilateral lobes of the prostate were dissected under aseptic conditions for paraffin inclusion and HE staining.Result The positive reaction in group M and each group T was significantly more than those in group C(P<0.001)one week after the modeling was completed,and there was no significant difference between group M and group T.The number of positive reactions in group T2 was lower than that in group M at each time point af
作者
金晨
卞子辰
宋正尧
王志平
冯彬
张力
郝宗耀
樊松
杨诚
刘祎
梁朝朝
Jin Chen;Bian Zichen;Song Zhengyao;Wang Zhiping;Feng Bin;Zhang Li;Hao Zongyao;Fan Song;Yang Cheng;Liu Yi;Liang Chaozhao(Department of Urology,The First Affiliated Hospital of Anhui Medical University,Hefei 230022,China;Department of Urology,Second Hospital of Lanzhou University,Lanzhou 730030,China)
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2020年第10期779-783,共5页
Chinese Journal of Urology
关键词
前列腺炎
低强度体外冲击波
慢性前列腺炎
治疗
Prostatitis
Low-intensity extracorporeal shock wave
Chronic prostatitis
Treatment
