摘要
炭疽病是鹅掌柴发生最严重的病害之一。本文从四川省成都市采集疑似为炭疽病的鹅掌柴病叶,进行了分离培养,获得培养形态完全不同的2株炭疽菌,致病性测定发现它们都为致病菌。多基因(核糖体内转录间隔区、肌动蛋白、3-磷酸甘油醛脱氢酶、几丁质合成酶、β-微管蛋白、钙调蛋白)的系统发育分析发现,分离获得的2株炭疽菌分别与暹罗炭疽菌(Colletotrichum siamense)和喀斯特炭疽菌(C.karstii)聚在一起,形成一个明显的分支,结合形态学特点,确定引起成都市鹅掌柴炭疽病的病原菌为暹罗炭疽菌和喀斯特炭疽菌,这是关于这两种病原引起鹅掌柴炭疽病的首次报道。本文还研究了不同的回接方式对致病性测定的影响,砂纸擦拭后选取中老叶片用孢子悬浮液接种是对鹅掌柴炭疽病最好的致病性测定方法。
Anthracnose is one of the most serious diseases of Scheffiera octophylla. In this study, two different types of strains were isolated from leave samples with symptoms of anthracnose of S. octophylla collected from Chengdu, Sichuan. Following infection assay of the plant suggested both strains to be the pathogenic agents that were identified as Colletotrichum siamense and C. karstii based on morphological, cultural characteristics and phylogenetic analyses of multi-locus sequences （intemal transcribed spaces, ITS ; actin, ACT; glyceraldehydes- 3-phosphate dehydrogenase, GAPDH; chitin synthase 1, CHS-1; fl-tubulin gene,TUB2; calmodulin, CAL）. This report is the first description of the two pathogens responsible for S. octophylla anthracnose. We also studied the influence of different inoculation methods and found that pretreatment by rubbing with sandpaper prior to spray conidial suspension on the old leaf was the best way for the pathogenicity test on S. octophylla.
出处
《植物病理学报》
CAS
CSCD
北大核心
2017年第3期296-304,共9页
Acta Phytopathologica Sinica
基金
四川农业大学双支计划(03572101)
四川农业大学导师培育计划(04051835)
关键词
鹅掌柴
暹罗炭疽菌
喀斯特炭疽菌
多基因
致病性
Scheffiera octophylla
Colletotrichum siamense
Colletotrichum karstii
multi-locus sequences
pathogenicity
