摘要
目的 :探讨石蜡切片免疫组织化学(免疫组化)检测肾穿刺异常免疫复合物的可行性。方法 :收集36例肾小球肾炎的穿刺活检标本,分别行冷冻切片免疫荧光、石蜡切片免疫组化检查,并以荧光免疫检测结果作为对照,观察免疫组化染色结果。结果:7例狼疮性肾炎、16例IgA肾病、13例膜性肾小球肾炎的免疫组化IgA、IgG、IgM、C1q、C3c、C4c结果与免疫荧光冷冻切片组染色结果相同,表达强度相同,表达部位一致(P>0.05)。结论 :经适当抗原修复,石蜡切片免疫组化检查可以作为免疫荧光冷冻切片结果不理想的补救方法。
Objective： To study the feasibility of detecting abnormal immune complex in paraffin section of renal core biopsy specimen by immunohistochemical method. Methods： Thirty-six core biopsy specimens of kidney with glomerular nephritis were examined by frozen section immunofluorescence and paraffin section immunohistochemistry to evaluate the results of immunohistochemical detection in reference with the that of immunofluorescence. Results＂ For the 7 cases of lu- pus nephritis, 16 cases of IgA nephropathy, 13 cases of membranous glomerular nephritis, immunohistochemical staining of IgA, IgG, IgM and Clq, C3c, C4c were in concordant with those of immunofluorescence both in expression intensity and location. Conclusions： Immunohistochemical examination of paraffin section after appropriate antigen retrieval can be used as a complementary method for the unsatisfactory result of immunofluorescence detection in frozen section.
出处
《诊断学理论与实践》
2017年第1期79-83,共5页
Journal of Diagnostics Concepts & Practice
关键词
肾活检
冷冻切片
石蜡切片
免疫荧光
免疫组织化学
Renal core biopsy
Frozen section, Paraffin section, Immunofluorescence
Immunohistochemistry
