摘要
本研究对水溶醇沉法提取的鸡腿菇粗多糖脱蛋白方法进行了比较,最终确定Sevage法为最优的脱蛋白方法。经DEAE-纤维素52离子交换及Sephadex G-200分子层析柱分级、纯化,最终得到2个主要的多糖组分Ccp-I-A和Ccp-I-B。理化性质测定结果表明,二者均为白色絮状固体,能溶于水,不溶于无水乙醇、丙酮等有机溶剂;与斐林试剂,CTAB、硫酸-咔唑、碘-碘化钾及三氯化铁反应均为阴性。GC测定结果可知Ccp-I-A主要由甘露糖、葡萄糖和半乳糖组成,摩尔比为2.03∶9.52∶1;Ccp-I-B主要由岩藻糖和半乳糖组成,其摩尔比为1∶5.21。另外,Ccp-I-A和Ccp-I-B对DPPH和·OH显示出良好的清除能力,而且,相比于Ccp-I-B,Ccp-I-A的清除能力更高,当浓度为300μg/mL,其对DPPH和·OH的清除能力可分别达到72.1%和55.3%。
We compared the ways of deproteinization for crude polysaccharides of Coprinus comatus, and finally selected Sevage method as the optimal method. Two main fractions of Ccp-l-A and Ccp-I-B were obtained after DEAE-52 cellulose and Sephadex G-200 chromatography, both were white-floc, soluble in water, insoluble in absolute ethyl alcohol, acetone and other organic solvents. Additionally, Fehling reagent, CTAB, Sulphuric acid-carbazole, I-KI and FeCl3 reaction were all negative. GC analysis showed Ccp-I-A was composed of mannitose, glucose and galactose in molar ratios of 2.03：9.52：1, whereas Ccp-I-B was composed of fucose and galactose with molar ratios of 1：5.21. Antioxidant activity test showed that Ccp-I-A and Ccp-I-B had good scavenging abilities on DPPH and -OH. Compared to Ccp-I-B,the scavenging activity of Ccp-I-A was much stronger, and the scavenging rate could reach 72.1% and 55.3% respectively when the concentration was 300μg/mL.
出处
《生物工程学报》
CAS
CSCD
北大核心
2017年第5期808-816,共9页
Chinese Journal of Biotechnology
基金
山西省科技重点研发计划项目(No.2015-TN-10)资助~~
关键词
鸡腿菇多糖
纯化
理化性质
抗氧化
Coprinus comatus polysaccharides, purification, physico-chemical property, antioxidation
