摘要
[目的]为了得到不能产生任何有活性的THC基因型大麻株系,本试验对四氢大麻酸(THCA)合成酶基因进行克隆和生物信息学分析,这为深入研究CsTHCA的功能提供理论基础。[方法]以大麻品种"火麻一号"为材料,采用RT-PCR技术克隆THCA合成酶基因(CsTHCA)并对其进行生物信息学分析。[结果]该基因开放阅读框全长1 638bp,编码545个氨基酸。推导的氨基酸序列在309~760bp处与野生种花生和蔓花生序列一致性达74%。理化特性分析发现此蛋白为疏水蛋白且结构稳定。蛋白结构功能域分析预测该蛋白质序列中有跨膜区域,大部分组成为疏水性氨基酸。[结论]本文从大麻中克隆了THCA基因并对其进行了生物信息学分析,可为后续分子机制的研究提供理论依据。
[Objective] The test was to obtain hemp strain which do not contain any THC. Cloning and bioinformatics analysis of THCA synthase gene could be helpful to facilitate the functions understanding of hemp THCA synthase. [-Methods] The full-length THCA synthase cDNA sequence was cloned using RT-PCR from hemp variety ＂Huoma 1＂ and CsTHCA coding protein was analyzed by bioinformatics software. [-Results] The open reading frame of CsTHCA was 1638bp in length and encoded 545 animo acids. The sequence homology analysis showed that CsTHCA protein had 74% similarity with that of wild Arachis ipaensis and Arachis duranensis at the location of amino acids 309 bp to 760 bp. Physical and chemical properties analyzing revealed that this protein was hydrophobic protein with stable charac- ter. The analysis of protein structure and function domain indicated that this protein had transmembrane region, and most amino acids were hydrophobic. [Conclusion] The CsTHCA was cloned from hemp and was analyzed by bioinfor-matics. That could provide a theoretical basis for the study of the molecular mechanism.
出处
《山西农业大学学报（自然科学版）》
CAS
2017年第5期326-329,365,共5页
Journal of Shanxi Agricultural University(Natural Science Edition)
基金
黑龙江省青年科学基金(QC2016037)
关键词
大麻
CsTHCA
基因克隆
生物信息学
Cannabis sativa L. , CsTHCA, Gene clone, Bioinformatics analysis
