摘要
目的:探讨不同剂量的乙肝病毒(HBV)对新生树鼩感染的影响。方法:将46只树鼩随机分为3组,分别接种含乙肝病毒10~2~10~3 copies/mL(低剂量组,n=14)、10~4~10~6copies/mL(中剂量组,n=16)和10~7~10~8 copies/mL(高剂量组,n=16)的感染者血清。定期采血检测乙肝标志物及HBV-DNA,采用电化学发光免疫测定法(ECLIA)检测乙肝两对半,PCR-荧光探针法检测外周血HBV-DNA。定期肝活检手术取肝组织进行病理检查。结果:观察周期内,低、中、高剂量组的HBsAg阳性率分别为100.0%、50.0%、31.2%,低剂量组显著高于其他两组(χ~2=15.322,P=0.000);低剂量组在8周、10周、18周的HBsAg阳性率大于其他两组(P<0.05);第24周,高剂量组的HBsAg阳性率大于其他两组(P<0.05)。在观察周期内,低、中、高剂量组的HBV-DNA阳性率分别为21.4%、6.2%、62.5%,高剂量组与其他两组比较,差异有统计学意义(χ~2=12.727,P=0.003)。HBsAg及HBV-DNA阳性的树鼩肝组织仅见轻微炎性改变。结论:不同剂量的HBV对新生树鼩感染模型有显著影响,高剂量的HBV更利于延长感染时间。
Objective.To investigate the effect of different HBV doses on the outcome of HBV infection in newborn tree shrews. Methods：46 tree shrews were randomly divided into three groups and injected with serum from HBV infectors. The virus titers were 10^2-10^3, 10^4-10^6 and 107-108 copies/mL in low- （ n = 14）, middle- （ n =16） and high-dose （ n =16） HBV groups, respectively. Peripheral blood was collected regularly for detecting HBV infection markers and HBV-DNA virus load. HBV infection markers were detected by electrochemiluminescence immunoassay （ECLIA）. HBV-DNA in peripheral blood was detected by PCR-fluorescence probe method. The liver biopsy specimens were taken for histopathological examination regularly. Results： The positive rates of HBsAg were 100.0%, 50. 0% and 31.2% respectively in low-, middle- and high-dose HBV groups, which was higher in the low-dose group than in other groups （x2=15. 322, P =0.000）. The positive rates of HBsAg at 8, 10 and 18 weeks in low-dose HBV group were higher than those in high- and middle-dose groups （ P 〈0. 05）, and were higher in high-dose HBV group at 24 weeks than in the low- and high-dose groups （ P 〈0.05）. The positive rates of HBV-DNA were 21. 4% , 6.2 % and 62.5% respectively in low-, middle- and high-dose HBV groups, which was higher in the high-dose group than the other two groups （X2= 12. 727, P =0. 003）. Mild inflammatory changes in liver tissues were observed in HBsAg and HBV-DNA positive tree shrews. Conclusion.. Different doses of HBV might be closely related to the outcome of HBV infection in newborn tree shrews, and high-dose HBV could prolong the duration of infection.
出处
《广西医科大学学报》
CAS
2017年第5期646-649,共4页
Journal of Guangxi Medical University
基金
国家自然科学基金资助项目(No.81460418)
