摘要
目的对自然妊娠与辅助生殖技术(ART)术后妊娠胚胎停育患者绒毛细胞染色体进行研究,分析自然妊娠与ART术后妊娠绒毛细胞染色体异常率,并通过二者之间的比较,从遗传学角度来验证ART手术是否安全。方法通过搜集患者绒毛组织,按照常规方法制备染色体涂片,通过G显带法进行染色体核型分析,并应用SPSS 13.0软件进行统计分析,比较2组间绒毛细胞染色体异常率的差异。结果异常核型共计145例,异常核型检出率为56.20%(145/258),以常染色体三倍体为主。自然流产绒毛细胞正常核型为63例,异常核型为73例,异常率为53.67%(73/136)。ART组正常核型为50例,异常核型为72例,异常率为59.01%(72/122)。自然流产组与ART组绒毛染色体异常率比较,差异无统计学意义(P>0.05)。结论早期胚胎停育与胚胎染色体异常密切相关,自然妊娠与ART术后妊娠绒毛细胞染色体异常率无显著差异。
Objective To study the villus chromosomal abnormalities in early spontaneous abortion after natural pregnancy and assisted reproductive technology（ART） in Jining for the first time, and assessed the freqency of the villus chromosomal abnormalities in the two group. Then we checked the safety of ART through the compare of the rates of the two group. Methods Villus tissues were collected and chromosome smears were prepared by routine methods. Karyotype analysis was performed by G banding method, and SPSS 13.0 software was used for statistical analysis. The differences of chromosome abnormality rate between two groups were compared. Results A total of 145 cases of abnormal karyotype, abnormal karyotype detection rate was 56.20% （ 145/258）, main of which were autosomal triploid. The normal karyotype of spontaneous abortion was 63 cases, the abnormal karyotype was 73 cases, and the abnormal rate was 53.67% （73/136）. In group ART, 50 cases had normal karyotype, and abnormal karyotype was found in 72 cases, and the abnormal rate was 59.01% （72/122）. There was no significant statistical difference between the groups of spontaneous abortion group and ART group in abnormal chromosome abnormality（P 〉 0.05）. Conclusion There was a close relationship between the early spontaneous abortion and the chromosomal abnormalities, but there was no significant difference in the chromosome abnormality rate between the natural pregnancy and ART after pregnancy.
作者
陈新科
周建伟
张昭才
常虹
郭焕
颜金艳
CHEN Xin- ke ZHOU Jian- wei ZHANG Zhao- cai CHANG Hong GUO Huan YAN Jin- yan(Affiliated Hospital of Ji＇ning Medical College, Ji＇ning, Shandong 272200, Chin)
出处
《中国卫生检验杂志》
CAS
2017年第9期1331-1333,共3页
Chinese Journal of Health Laboratory Technology
基金
济宁医学院附属医院苗圃科研基金项目(MP-2014-)
关键词
胚胎停育
辅助生殖技术
自然妊娠
绒毛细胞染色体
Early spontaneous abortion
Assisted reproductive technology
Natural pregnancy
Villus chromosome
