摘要
钙信号动态变化在植物适应非生物胁迫中起着重要作用。为了理解热胁迫下1个微丝结合相关蛋白(Actin-Related Protein 2,ARP2)参与调控钙信号的机制,采用生理、分子和遗传手段对ARP2相关遗传材料进行了分析。实验结果发现,和野生型相比,热胁迫下arp2突变体细胞质的钙离子浓度升高,arp2突变体幼苗表现出热敏感表型。ARP2基因的表达受热胁迫的诱导,arp2突变体中热诱导响应的转录因子HSF-A7A、HSF-B2A的表达较野生型低。当外源添加线粒体渗透性转换孔道抑制剂cyclosporin A(CsA)抑制arp2突变体中钙升高时,可以恢复其热敏感的表型。以上研究结果表明,ARP2通过调控线粒体来源的钙信号响应热胁迫。
The dynamic change of calcium signal plays an important role in plant adaptation to abiotic stresses.In order to understand the mechanism of ARP2 mediated Ca^2＋signal under heat stress,we use physiological,molecular and genetic tools to analysis the ARP2 genetic material.The results show that plant lacking ARP2 exhibits enhanced heat-induced increases in[Ca^2＋]cyt,and hypersensitivity to heat stress.The expression of ARP2 is induced under heat stress,and the mRNA expression level of several HSFs such as HSF-A7 A,HSF-B2 Ais reduced in arp2 mutant.When addition of cyclosporin A（CsA）,an inhibitor of mitochondrial permeability transition pore,the increase of[Ca^2＋]cyt is prevented,and the heat-sensitive phenotype of the arp2 mutant is rescued.These results indicate that the ARP2 regulates mitochondrial-dependent Ca^2＋ signaling in response to heat stress.
出处
《中国科技论文》
北大核心
2017年第6期720-724,共5页
China Sciencepaper
基金
高等学校博士学科点专项科研基金资助项目(20120008120004)
国家自然科学基金资助项目(31670260)
转基因生物新品种培育重大专项(2016ZX08009002)
关键词
拟南芥
热胁迫
钙信号
ARP2蛋白
arabidopsis
heat stress
calcium signaling
ARP2 protein
