摘要
目的:研究转录因子Twist和上皮细胞钙黏蛋白(E-cadherin)在口腔鳞状细胞癌(OSCC)中的表达,探讨其与OSCC发生发展的关系。方法:采用免疫组化SP法分别检测Twist和E-cadherin在20例正常口腔黏膜组织和78例OSCC组织中的表达,用Spearman秩相关分析Twist和E-cadherin在OSCC组织中表达的相关性。结果:Twist在OSCC组织中的表达明显高于正常口腔黏膜组织(P<0.05);E-cadherin蛋白在OSCC组织中的表达明显低于正常口腔黏膜组织(P<0.05);E-cadherin在Ⅲ、Ⅳ期OSCC组织中的表达低于Ⅰ、Ⅱ期(P<0.05);E-cadherin在淋巴结转移患者的OSCC组织中的表达低于无转移者(P<0.05);Twist在Ⅲ、Ⅳ期OSCC组织中的表达高于Ⅰ、Ⅱ期(P<0.05);Twist在淋巴结转移患者的OSCC组织中的表达高于无转移者(P<0.05);OSCC组织中Twist的表达与E-cadherin的表达呈显著负相关(r=-0.639,P<0.05)。结论:Twist与E-cadherin在OSCC组织中异常表达,且两者的表达高度相关,提示两者可能参与OSCC的发生、发展、浸润和转移,联合检测Twist和E-cadherin对OSCC的预防诊治及预后评估具有较大的临床价值。
Objective： To investigate the expression of transcript ion factor Twist and epithel ial cel l calcium mucin （E - cadherin） in oral squamous cell carcinoma（ OSCC） and to explore their relationship between with the occur-rence and development of OSCC. Methods ： Immunohistochemistry SP method was used to detected the expression of Twist and E - cadherin in 20 cases of normal oral mucosa tissues and 78 cases of OSCC tissues. Correlation between expression of Twist and E - cadherin in OSCC was analysed by Spearman rank correlation analysis. Results： The ex-pression of Twist in OSCC tissues was significantly higher than normal oral mucosa tissues （ P 〈 0. 05 ） . The expression of E - cadherin in OSCC tissues was significantly lower than normal oral mucosa tissues（ P 〈 0. 05 ） . The expression of E - cadherin in OSCC tissues of stageⅢ and Ⅳ was lower than stage Ⅰ and Ⅱ （P 〈 0 . 05 ） . The expression of E - cadherin in OSCC tissues of patients with lymph node metastasis was lower than that non metastasis（ P 〈 0. 05 ） . The expression of Twist in OSCC tissues of stage Ⅲ and Ⅳ was higher than stage Ⅰand Ⅱ （P 〈 0. 05 ） . The expression of Twist in OSCC tissues of patients with lymph node metastasis was higher than that non metastasis （P 〈0.05）. The ex-pression of Twist in OSCC was negatively correlated with the expression of E - cadherin（r =-0. 639 ,P 〈0.05 ） . Conclusion：The abnormal expression of Twist and E - cadherin in OSCC tissues,and their expression was highly cor-related, suggesting that they may participate in the occurrence and development of OSCC, invasion and metastasis. Combined detection of Twist and E - cadherin has great clinical values in the prevention, diagnosis, treatment and prognosis of OSCC.
出处
《现代肿瘤医学》
CAS
2017年第12期1884-1887,共4页
Journal of Modern Oncology
基金
宜宾市卫生局科学技术计划基金资助项目(编号:[2014]350号)
