摘要
【目的】16SrI组植原体对我国作物和生态植物危害严重。目前植原体尚不能分离培养,对于我国植原体不同株系间遗传变异和种群结构尚不清晰,通过多位点序列分析(MLSA)揭示我国不同地区16SrI组不同植原体株系的遗传多样性及其地理分布特征,比较不同植原体株系间不同持家基因的变异程度,为我国不同植原体株系的检测、分类鉴定和系统发育研究提供一定的参考方法和依据。【方法】应用rp,tuf,secA,secY,ipt,dnaK,fusA,gyrB,pyrG和rpoB共10个持家基因序列,结合16S rDNA序列,以全基因序列已完成的洋葱黄化植原体(OY-M)、翠菊黄化丛枝植原体(AYWB)、澳大利亚葡萄黄化植原体(CPA)、草莓致死黄化植原体(SLY)和苹果簇生植原体(CPM)共5种植原体株系为参照,分析我国10个省(市)苦楝丛枝、莴苣黄化、桑萎缩、泡桐丛枝和长春花绿变植原体株系(共18株)的遗传变异规律和系统发育关系,通过多重序列比对分析不同基因片段的序列多态性和变异程度。【结果】我国18株苦楝丛枝、莴苣黄化、桑萎缩、泡桐丛枝和长春花绿变植原体株系的rp,tuf,secA,secY,ipt,dnaK,fusA,gyrB,pyrG和rpoB多位点序列共有15种序列类型,从而揭示16SrI组不同植原体株系间丰富的遗传多样性。基于rp等10个基因多位点序列分析可将16SrI-B、D亚组的不同植原体株系清晰地分开。10株苦楝丛枝植原体株系与2株桑萎缩植原体株系系统发育关系最近,多位点序列分析可将这2种基于16S rDNA序列难以区分的植原体株系清晰地区分。10株苦楝丛枝植原体株系分为4个进化枝,其多位点序列存在8种序列类型,这4个分枝与植原体株系的地理分布关系密切。与我国长春花绿变和泡桐丛枝植原体株系相比,福建三明2株莴苣黄化植原体株系与日本洋葱黄化植原体株系OY-M系统发育关系较近。在已检测分析的植原体不同基因序列片段中,dnaK基因�
[ Objective] The phytoplasmas in 16SrI group cause severe diseases of many crops and ecological plants in China. At present, the genetic variation and population structure of the yet-uncuhured and very closely related phytoplasma strains in China are still not fully understood. A multilocus sequence analysis （MLSA） scheme was used to elucidate genetic diversity of phytoplasma strains in 16SrI group from different regions in China and their relationships with geographical distribution, and to compare the levels of variation in different housekeeping gene of different phytoplasma strains. The study aimed to provide some reference approach and evidence for finer detection, identification, classification and phylogenetic analysis of different phytoplasma strains in China. [ Method] Ten housekeeping gene （rp, tuf, secA, secY, ipt, dnaK, fusA, gyrB, pyrG and rpoB） fragments combined with 16S rDNA sequences were employed to analyze the genetic variation and phylogenetic relationships of 18 phytoplasma strains infecting chinaberry, lettuce, mulberry, paulownia and periwinkle from ten provinces in China, using five phytoplasma strains, onion yellows phytoplasma （ OY- M）, aster yellows witches＇ -broom phytoplasma （ AYWB）, Candidatus Phytoplasma australiense （ CPA）, strawberry lethal yellows phytoplasma （SLY） and Candidatus Phytoplasma mali （CPM）, of which whole genomes have been sequenced, as references. Sequence polymorphism and variation levels of different gene fragments were analyzed by multiple sequence alignment. [ Result ] The nucleotide site polymorphisms of rp, tuf, secA, secY, ipt, dnaK, fusA, gyrB, pyrG and rpoB gene fragments were used to resolve all strains into 15 sequence types （STs）, demonstrating extensive genetic diversity among the 16SrI group strain population. All the strains, classified into 16SrI-B and -D subgroup by 16S rDNA analysis, were clustered into one clade and clearly differentiated into discrete subclades by phylogenetic analysis of the concatenated gene se
出处
《林业科学》
EI
CAS
CSCD
北大核心
2017年第3期105-118,共14页
Scientia Silvae Sinicae
基金
The National High Technology Research and Development Program of China(2012AA101501)
National Infrastructure of Microbial Resources of China(NIMR2014-7)
