摘要
【目的】探明高丹草杂种及其亲本叶片基因差异的多样性。【方法】以4份高粱不育系和5种类型苏丹草为亲本,按照NCⅡ设计组配成20个杂交组合,测量各杂交组合及亲本的田间形态指标和生理生化指标,采用cDNA-AFLP差异显示技术分析其基因差异多样性,计算29份材料的遗传相似系数并进行UPGMA聚类分析。【结果】20份高丹草杂种及其9份亲本材料的cDNA-AFLP分析结果显示,12对引物组合共扩增出336个位点,其中特异性位点276个,占总数的82.14%,平均每对引物组合扩增出特异性位点23个。29份材料的观测等位基因数(No)为1.870 1,有效等位基因数(Ne)为1.736 5,Nei’s基因多样性指数(H)为0.389 8,Shannon信息指数(I)为0.556 9,显示了高丹草杂种及其亲本苗期叶片基因表达的复杂性。聚类分析结果显示,29份材料间的遗传相似系数为0.508~0.876,以遗传相似系数0.54为基准,可将29份材料分为4类,其中亲本材料主要在第Ⅰ类和第Ⅳ类,杂种主要在第Ⅱ类和第Ⅲ类。【结论】构建了高丹草cDNA-AFLP差异显示技术反应体系,表明高丹草及其亲本苗期叶片基因具有差异多样性。
[Objective] This study investigated the genetic diversity differences in leaves of hybrids and parents of Sorghum-sudangrass. [Method] Four sorghum sterile lines and five Sudan grass were used as parents to formulate 20 hybrid combinations based on the NC Ⅱ design. Field shape indexes and physiological and biochemical indexes of the hybrids and parents were measured. Genetic diversity differences were analyzed using cDNA-AFLP differential display technique,genetic similarity coefficients of the 29 materials were calculated, and cluster analysis was conducted. [Result] The cDNA-AFLP analysis of 29 sorghum hy- brids and their parents showed that 12 pairs of primer combinations amplified 336 sites,including 276 spe- cific sites with the ratio of 82. 14%. The average specific sites of each primer combination was 23. The number of alleles （No） was 1. 870 1, the effective number of alleles （Ne） was 1. 736 5, the Nei ＇ s gene di-versity index （H） was 0. 389 8, and the Shannon （I） was 0. 556 9, indicating the complexity in gene expres- sion of the hybrids and parents. Cluster analysis showed that the genetic similarity coefficients of 29 Sor- ghum-sudangrass materials were 0. 508-0. 876. Using 0.54 as standard,the 29 materials can be divided in- to 4 categories. The parental materials were mainly in types I and IV ,and the hybrids were mainly in types Ⅱ and Ⅲ. [Conclusion] The reactive system of cDNA-AFLP differential display technique was estab- lished,and it showed that the leaves of Sorghum-sudangrass hybrids and their parents had genetic diversity at seedling stage.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2017年第5期48-56,共9页
Journal of Northwest A&F University(Natural Science Edition)
基金
国家自然科学基金项目(31460375)
呼和浩特市科技计划项目(2012-重-计-8-2)
