摘要
用RAPD分子标记对12个无芒雀麦种群进行遗传多样性分析,结果表明:选用的9条多态性引物共扩增出87个条带,平均每个引物扩增9.67条,其中多态性条带45个,多态性条带百分率为51.72%。无芒雀麦物种水平上的多态性条带数为76个,多态性条带百分率为87.36%,Shannon信息指数为0.2933,Nei’s基因多样性指数为0.1843。种群间遗传分化系数为0.2735,说明无芒雀麦的遗传分化主要发生在种群内。聚类结果显示,部分种群间的遗传距离与地理距离有一定的相关性,但也有例外,这可能与人类的广泛栽培加大了种群间的基因交流有关。
Random amplified polymorphic DNA（RAPD） techniques were used to detect the genetic diversity of 12 Bromus inermis Leyss populations.A total of 87 bands were amplified with 9 RAPD primers,of which 45 were polymorphic bands.The average percentage of polymorphic bands were 51.72%.76 were polymorphic（PPB=87.36%） in species level.The results of Popgen32 analysis indicated that Nei＇s gene diversity（H） was 0.1843,Shannon information index（I＊） was 0.2933.Gene difference index（Gst） among populations was 0.2735,which means the percentage of gene difference among populations was only 27.35%.The results showed that the genetic variation within population was higher than among populations.The cluster analysis showed that genetic distance was related to geographic distance only on partial populations,human＇s wide cultivation maybe broaden the gene flows among populations.
出处
《中国草地学报》
CSCD
北大核心
2011年第2期25-30,共6页
Chinese Journal of Grassland
基金
国家草品种区域试验项目"草品种抗旱性鉴定"(2010)
国家科技支撑计划课题(2011BAD17B01)
中央级公益性科研院所基本科研业务费专项资金(中国农业科学院草原研究所)资助
关键词
无芒雀麦
种群
RAPD
遗传多样性
Bromus inermis Leyss
Population
RAPD
Genetic diversity
