摘要
提出了构建一种用于细胞非接触操作的激光微束系统。该系统由 Nd:YAG激光束经声压、热膨胀、汽化等综合效应实现的光刀和 He- Ne激光束经光学动力学效应实现的光镊组成。将两激光束耦合到显微镜中 ,实现了生物细胞的捕获、移动、翻转、打孔等一系列操作。在此基础上 ,分析了形成光镊所必需产生梯度力场的条件和形成光刀对能量的要求 ,进行了系统的总体设计、关键部件设计和选择 ,构建了一套激光微束操作实验系统 ,得到了预期的试验结果。在该系统上成功地实现了非接触细胞操作 ,并对染色体进行了切割。
A micro-beam laser system was constructed for cell manipulation. This system includes optical scissors and optical tweezers with optical kinetic effects. The system can manipulate, deflect, rotate, and punch cells when two laser beams are coupled into a microscope. The overall design of the laser micro-beam system is presented with the design of critical components for the required gradient force field for forming the optical tweezers and the energy needed to form the optical scissors. A micro-beam laser system is built that could successfully manipulate cells in suspension and could slice chromosomes.
出处
《清华大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2002年第8期1064-1067,共4页
Journal of Tsinghua University(Science and Technology)
基金
北京市自然科学基金资助项目 (3 0 0 2 0 0 7)
清华大学基础研究基金资助项目 (JC19990 12 )
关键词
激光微束
光刀
光镊
细胞操作
laser micro-beam
optical scissors
optical tweezers
cell manipulation
