摘要
目的了解温州地区不同来源蜡样芽胞杆菌毒力基因分布、生化分型和多位点序列分型(MLST)特征。方法参照GB 4789.14—2014对127株蜡样芽胞杆菌进行鉴定和生化分型,同时采用PCR方法检测10种毒力基因,并进行MLST基因分型。结果 127株蜡样芽胞杆菌同时携带Nhe A、Nhe B、Nhe C基因的菌株占94.5%(120/127),而同时携带hbl A、hbl C、hbl D基因的占9.4%(12/127),携带ces基因的占7.9%(10/127);127株菌株除10株不能进行生化分型外,其余117株分为2型(0.8%,1/127)、5型(3.9%,5/127)、8型(1.6%,2/127)、9型(63.8%,81/127)和10型(22.0%,28/127);通过MLST分析,72株菌株共分为28个ST序列型,ST26(18.1%,13/72)、ST144(15.3%,11/72)、ST92(6.9%,5/72)和ST164(6.9%,5/72)为常见ST型别。28个ST型聚类分析显示温州地区蜡样芽胞杆菌分为4个序列群和13个单态群。结论温州地区蜡样芽胞杆菌毒力基因携带率较高,遗传关系具有多样性。
Objective To investigate the virulence genes,biochemical typing and multilocus sequence typing（MLST）distribution of Bacillus cereus from different sources in Wenzhou.Methods 127 strains of Bacillus cereus were identified and biochemical typed according to GB 4789.14-2014.Ten virulence genes were detected with polymerase chain reaction（PCR）.Genetic typing was conducted with MLST.Results Of 127 isolates,Nhe A,Nhe B and Nhe C genes were accounted for 94.5%（120/127） and hbl A,hbl C and hbl D genes were accounted for 9.4%（12/127）.The emetic toxin gene was detected in 10 isolates（7.9%）.Except 10 isolates which could not be typed,the rest 117 isolates were classified into type 2（0.8%）,type 5（3.9%）,type 8（1.6%）,type 9（63.8%） and type 10（22.0%） by biochemical typing.Seventy-two isolates could be classified into 28 sequence types（STs）.ST26（18.1%）,ST144（15.3%）,ST92（6.9%） and ST164（6.9%） were the common STs.Cluster analysis on 28 STs generated four MLST groups and 13 singletons.Conclusion The positive rate of virulence genes was high in Bacillus cereus isolated from different sources in Wenzhou,and the genetic relationship was diverse.
作者
李毅
章乐怡
洪程基
胡玉琴
LI Yi ZHANG Le-yi HONG Cheng-ji HU Yu-qin(Wenzhou Center for Disease Control and Prevention, Zhejiang Wenzhou 325000, Chin)
出处
《中国食品卫生杂志》
2017年第2期172-176,共5页
Chinese Journal of Food Hygiene
