摘要
目的:比较四君子汤配方颗粒与传统汤剂中总皂苷和总多糖含量的差异。方法:采用紫外分光光度法在540 nm、488 nm波长处分别对四君子汤配方颗粒与传统汤剂中总皂苷和总多糖的含量进行测定。结果:人参皂苷Re在10.909~65.454μg·ml^(-1)浓度范围内与吸光度呈良好的线性关系(r=0.9997),加样回收率为98.49%,RSD为0.85%(n=6);D-无水葡萄糖在2.160~12.960μg·ml^(-1)浓度范围内与吸光度呈良好的线性关系(r=0.9997),加样回收率为99.46%,RSD为0.73%(n=6)。3个批次的配方颗粒供试品溶液中总皂苷的含量略高于传统汤剂,而总多糖的含量略低于传统汤剂,两者含量差异无统计学意义(P>0.05)。结论:四君子汤配方颗粒与传统汤剂间的物质基础差异较小,该配方颗粒应用于临床具有一定的可行性。
Objective： To compare the content of total saponins and total polysaccharides between formula granule and traditional decoction of Sijunzi decoction. Methods： UV spectrophotometry was used to determine the content of total saponins and total polysac-charides in formula granule and traditional decoction of Sijunzi decoction respectively at the wavelength of 540 nm and 488 nm. Restilts： The absorbance of ginsenoside Re had a good linear correlation with the concentration within the range of 10. 909-65. 454 μg· ml^ -1 （ r = 0. 999 7 ）, and the average recovery was 98.49% （ RSD = 0.85%, n = 6 ） ; the absorbance of D-anhydrous glucose had a good linear correlation with the concentration within the range of 2. 160-12. 960μg· ml^ -1 （r = 0. 999 7 ） , and the average recovery was 99.46% （ RSD = 0.73 % , n = 6）. The contents of total saponins from 3 batches of formula granule were slightly higher than those from traditional decoction, and that of total polysaccharides in formula granule was slightly lower than that in traditional decoction, and there was no significant difference between the two groups （ P 〉 0.05 ） Conclusion： The difference of material basis between formula granule and traditional decoction of Sijunzi decoction is small, and formula granule is more feasible for clinical application.
出处
《中国药师》
CAS
2017年第5期798-801,共4页
China Pharmacist
基金
浙江省中医药科技计划资助项目(编号:2016ZQ014)
关键词
四君子汤
配方颗粒
传统汤剂
总皂苷
总多糖
含量
Sijunzi decoction
Formula granule
Traditional decoction
Total saponins
Total polysaccharides
Content
